Study of biologically active substances of dry extract from the leaves of ordinary horse chestnut with high-performance liquid chromatography (Article)
a I.M. Sechenov First Moscow State Medical University, str. Trubetskaya 8, Moscow, Russian Federation
b Scientific Research Institute of Medicinal and Aromatic Plants (VILAR), Moscow, Russian Federation
b Scientific Research Institute of Medicinal and Aromatic Plants (VILAR), Moscow, Russian Federation
Abstract
The phenolic compounds, organic acids and sugars in the dry extract from the leaves of ordinary horse chestnut were studied with HPLC method. The qualitative composition has been determined and the content of certain groups of biologically active substances in the test object has been evaluated. Based on the obtained experimental data the researchers developed and unified the methodology of HPLC analysis of the dry extract from the leaves of ordinary horse chestnut. The development of qualitative and quantitative analysis was preceded by analytical work on the selection of the conditions of the study. Using the HPLC method with standard samples the authors have identified escin, 12 compounds related to phenolic compounds. The obtained results allowed offering reliable replicable methods of qualitati. nd quantitative analysis. © IDOSI Publications, 2013.
Author keywords
Escin; High performance liquid chromatography (HPLC); Medicinal plant material; Ordinary horse chestnut (Aesculus hippocastanum L.); Organic acids; Phenolic compounds; Sugars
Indexed keywords
EMTREE drug terms: Aesculus hippocastanum extract; ascorbic acid; caffeic acid; catechin; chlorogenic acid; cichoric acid; citric acid; epicatechin; epigallocatechin gallate; esculetin; ferulic acid; fructose; gallic acid; glucose; hyperin; lactic acid; luteolin 7 glucoside; malic acid; oxalic acid; tannin; tartaric acid
EMTREE medical terms: Aesculus hippocastanum; article; controlled study; drug determination; high performance liquid chromatography; nonhuman; phytochemistry; plant leaf; qualitative analysis; reliability
Chemicals and CAS Registry Numbers: ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; caffeic acid, 27323-69-9, 331-39-5; catechin, 13392-26-2, 154-23-4; chlorogenic acid, 327-97-9; cichoric acid, 6537-80-0; citric acid, 126-44-3, 5949-29-1, 77-92-9, 8002-14-0; epicatechin, 490-46-0; epigallocatechin gallate, 989-51-5; esculetin, 305-01-1; ferulic acid, 1135-24-6, 24276-84-4; fructose, 30237-26-4, 57-48-7, 7660-25-5, 77907-44-9; gallic acid, 149-91-7; glucose, 50-99-7, 84778-64-3; hyperin, 482-36-0; lactic acid, 113-21-3, 50-21-5; luteolin 7 glucoside, 5373-11-5; malic acid, 149-61-1, 6915-15-7; oxalic acid, 144-62-7; tannin, 1401-55-4; tartaric acid, 133-37-9, 3715-17-1, 526-83-0, 526-94-3, 87-69-4
ISSN: 19920075Source Type: Journal Original language: English
DOI: 10.5829/idosi.gjp.2013.7.3.1109Document Type: Article
