Thursday, 23 June 2016

Dietary α-linolenic acid from flaxseed oil improved folliculogenesis and IVF performance in dairy cows, similar to eicosapentaenoic and docosahexaenoic acids from fish oil

Volume 146, Issue 6, December 2013, Pages 603-614


The objectives of this study were to determine the differential incorporation of various omega-3 (n-3) fatty acids (FAs) supplemented to dairy cows into ovarian compartments and assess the effects on IVF. Forty-two 256-day pregnant cows were supplemented with encapsulated fats, in treatments designated as i) SFA - saturated fat at 240 and 560 g/day per cow, prepartum and post partum (PP) respectively; ii) FLX - flaxseed oil at 300 and 700 g/day per cow prepartum and PP respectively; and iii) FO - fish oil at 300 and 700 g/day per cow prepartum and PP respectively. Commencing at 60 days in lactation, ovum pickup (OPU) was performed twice weekly (20 sessions; five cows per group) and in vitro maturation and IVF were conducted. The proportion of α-linolenic acid (ALA) was greater in follicular fluid (FF), granulosa cells, and cumulus-oocyte complexes (COCs) of FLX cows than in other groups (P<0.001). The proportion of docosahexaenoic acid (DHA) was 6.7 times as great in FF of FO as in other groups (P<0.001); docosapentaenoic acid n-3 and DHA were detected in COCs of FO but not in others. The follicle number during OPU was higher in FLX and FO than in SFA (P<0.05), and the oocyte cleavage rate was higher in FLX and FO than in SFA (P<0.01). Also, the percentage of oocytes that developed to blastocysts tended to be higher in both n-3 groups than in SFA (P<0.1). In conclusion, both dietary n-3 FAs similarly improved folliculogenesis and IVF performance; therefore, ALA-rich botanical n-3 seems to be a satisfactory approach to improve oocyte quality. © 2013 Society for Reproduction and Fertility.

Indexed keywords

EMTREE drug terms: docosahexaenoic acid; fish oil; icosapentaenoic acid; linolenic acid; linseed oil
EMTREE medical terms: animal experiment; article; controlled study; cumulus cell; dairy cattle; fat intake; female; fertilization in vitro; granulosa cell; in vitro oocyte maturation; nonhuman; oocyte; ovary follicle development; ovary follicle fluid; priority journal
MeSH: alpha-Linolenic Acid; Animals; Cattle; Dairying; Dietary Fats; Docosahexaenoic Acids; Eicosapentaenoic Acid; Female; Fertilization in Vitro; Fish Oils; Linseed Oil; Ovarian Follicle; Pregnancy; Treatment Outcome
Medline is the source for the MeSH terms of this document.
Chemicals and CAS Registry Numbers: docosahexaenoic acid, 25167-62-8, 32839-18-2; fish oil, 8016-13-5; icosapentaenoic acid, 25378-27-2, 32839-30-8; linolenic acid, 1955-33-5, 463-40-1; linseed oil, 8001-26-1
ISSN: 14701626 CODEN: RCUKBSource Type: Journal Original language: English
DOI: 10.1530/REP-13-0244 PubMed ID: 24062566Document Type: Article

  Moallem, U.; Department of Ruminant Science, Institute of Animal Sciences, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel;
© Copyright 2013 Elsevier B.V., All rights reserved.