J Parasit Dis. 2016 Sep;40(3):643-7. doi: 10.1007/s12639-014-0550-3. Epub 2014 Sep 5.
- 1Pharmacology Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
- 2Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
- 3Shahid Bahonar University of Kerman, Kerman, Iran.
- 4Department
of Food Hygiene and Public Health, Faculty of Veterinary Medicine,
Shahid Bahonar University of Kerman, Kerman, Iran ; Research Center for
Modeling in Health, Institute for Futures Studies in Health, Kerman
University of Medical Sciences, Kerman, Iran.
Abstract
Because of developing resistance to the existing anthelmintic drugs, there is a need for new anthelmintic agents. Tobacco plant has alkaloid materials that have antiparasitic effect. We investigated the in vitro anthelminthic effect of aqueous and alcoholic extract of Tobacco (Nicotiana tabacum) against M. marshalli. For investigating this effect, we prepared three dilutions of aqueous and alcoholic extract of Tobacco
(25, 50 and 75 mg/ml). The worms exposed to extracts for 10 h at
25-30 °C. The buffer PBS used as negative control and 50 mg/ml dilution
of Levamisole used as standard reference. In each group, 50 worms were
examined. We used an inhibition mobility test for our study. Survival
analysis with Cox proportional hazard model was used for data analysis.
The result showed that compared with Levamisole 50 mg/ml, dilution of 25
and 50 mg/ml of the aqueous extract had the same anthelminthic effects (P > 0.05), but 75 mg/ml dilution of the aqueous extract and dilution of 25, 50 and 75 mg/ml of alcoholic extract had more anthelminthic effect (P < 0.05). Overall, extracts of Tobacco
possess considerable anthelminthic activity and more potent effects
were observed with the highest concentrations. Therefore, the in vivo
study on Tobocco in animal models is recommended.
KEYWORDS:
Anthelmintic; Levamisole; Marshallagia marshalli; Tobacco
