- 1Chemistry Department, Institute of Exact Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
- 2Department
of Physiology and Biophysics, Institute of Biological Sciences,
Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais,
Brazil.
- 3Pharmacy Department, Universidade Federal de São João del Rey, São João del Rey, Minas Gerais, Brazil.
- 4Department
of Restorative Dentistry, Faculty of Dentristry, Universidade Federal
de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Abstract
The present work demonstrated an efficient cutaneous wound healing using Bixin-loaded polycaprolactone (PCL) nanofibers as a controlled delivery system. The influence of Bixin (Bix) content on PCL
nanofiber, Bix-PCL1(2.5% w/w bix) and Bix-PCL2 (12.5% w/w bix)
formation was investigated using electrical conductivity, attenuated
total reflectance infrared spectroscopy, X-ray diffraction, thermal
analysis, and scanning electronic microscopy. The results showed that a
greater bixin concentration resulted in higher polymeric solution
electrical conductivity. Moreover, higher polymeric solution electrical
conductivity provides lower nanofibers in terms of average diameter than pure PCL nanofibers.
In vitro release was largely governed by a diffusion-controlled
mechanism. The initial Bixin release domain showed a burst release over
the first 10 hours where approximately 30% and 40% of Bixin was released
from Bix-PCL1 and Bix-PCL2 nanofibers,
respectively. The second kinetic domain was comprised of a continuous
and slow Bixin release that led to almost 100% of the Bixin being
released within 14 days. The results on excisional wound model in induced diabetic mice indicated that the low concentration of Bixin released from loaded Bix-PCL nanofibers maintain the biological activity of Bixin and is efficient in accelerating the wound healing as well as in reducing the scar tissue area compared with pure PCL nanofibers. Therefore, soft material Bixin-loaded PCL nanofibers are a promising candidate for use in wound dressing. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2016.
© 2016 Wiley Periodicals, Inc.
KEYWORDS:
bixin; diabetes mellitus; polycaprolactone (PCL); polymeric nanofiber; wound healing
