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Journal of Chemical and Pharmaceutical Research
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ISSN No: 0975-7384
CODEN(USA): JCPRC5
J. Chem. Pharm. Res., 2010, 2(3):295-303
295
Evaluation of biochemical contents, nutritional val
ue, trace
elements, SDS-PAGE and HPTLC profiling in the leave
s of
Ruellia
tuberosa
L.
and
Dipteracanthus patulus
(Jacq.)
A.
Manikandan
#
and D. Victor Arokia Doss*
Department of Biochemistry, PSG College of Arts and
Science, Coimbatore, Tamil Nadu, India
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___________________________
ABSTRACT
The present study was conducted to investigate the
presence of biochemical contents, trace
elements, nutritive value evaluation and determinat
ion of molecular weight of proteins by SDS-
PAGE and phytochemicals detection by HPTLC in the l
eaves and 50% hydroethanolic leaf
extracts of Ruellia tuberosa L. and Dipteracanthus
patulus (Jacq.). The biochemical contents,
trace elements, nutritive value were determined by
different biochemical methods, trace elements
presence was detected by using Atomic Absorption Sp
ectroscopy (AAS), while the proteins and
phytochemicals were detected by using SDS-PAGE and
HPTLC. Ruellia tuberosa L. and
Dipteracanthus patulus (Jacq.) leaves confirmed the
presence of flavonoids, glycosides, phenols
saponins and showed minimum amount of trace element
s with moderate nutritive value. Vitamins
(E, C), total phenolics, carotenoid content and nut
ritive value were found to be greater in the
leaves of Ruellia tuberosa L. The protein bands obt
ained in the SDS-PAGE was found to be
similar for both the plant leaves. Our findings sug
gest that leaves of Ruellia tuberosa L. and
Dipteracanthus patulus (Jacq.) is endowed with anti
oxidant phytochemicals and moderate
nutritive value could serve as a base for future dr
ugs.
Keywords:
Ruellia tuberosa
L.,
Dipteracanthus patulus
(Jacq.), Nutritive value
