int J Biol Macromol. 2016 Oct;91:1110-9. doi: 10.1016/j.ijbiomac.2016.06.075. Epub 2016 Jun 23.
- 1Department of Pharmaceutical Science, Suresh Gyan Vihar University, Jaipur, RJ, India.
- 2Department
of Pharmaceutical Sciences, Dr. H. S. Gour Central University, Sagar,
MP, 470003, India. Electronic address: sushilkkashaw@gmail.com.
- 3Department of Pharmacy, RKDF College of Pharmacy, SRK University, Bhopal, MP, India.
- 4Department of Pharmacy, Guru Ramdas Khalsa Institute of Science & Technology, Jabalpur, MP, India.
Abstract
The
Apigenin (APN) was isolated from ethanolic extract of M. alba leaves
and screened by in-vivo wound models (Diabetic and Dead space) in rats.
Apigenin loaded hydrogel (HGs) was prepared using gellan gum-chitosan
(GGCH) with PEG as a cross linker and characterized for various
parameter like AFM, swelling property, entrapment efficiency and drug
release. Further performance of hydrogel was evaluated by wound healing
activity tested against wound contraction, collagen content, dried
granuloma weights and antioxidant activity. The percent entrapment
efficiency of optimized hydrogel found to be 87.15±1.20. APN loaded
GGCH-HGs were able to release 96.11% APN in 24h. The level of superoxide
dismutase (SOD) and catalase were found increased significantly in
granuloma tissue of APN treated group. APN GGCH-HGs found higher wound
healing effect in diabetic as well as normal wound tissues with
significant antioxidant activity. Results proven the utility of prepared
hydrogel (APN loaded GGCH-HGs) seems to be highly suitable for wound
healing due to its unique properties of biocompatibility,
biodegradability, moist nature and antioxidant effectiveness.
Copyright © 2016 Elsevier B.V. All rights reserved.
KEYWORDS:
Antioxidant; Apigenin; Hydrogels; Morus alba; Wound healing
