Available online 3 February 2015
Anthelmintic activity of Ocimum sanctum leaf extract against ovine gastrointestinal nematodes in India
Highlights
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- In vitro and in vivo efficacy of extracts of Ocimum sanctum on sheep helminthosis.
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- Phytochemical studies to know the active ingredients.
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- Assessment of blood, enzymes and antioxidant parameters for safety trail.
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- Detection of significant anthelmintic efficacy.
Abstract
Leaves of Ocimum sanctum have been traditionally used for various ethno-veterinary practices as well as medicinal purpose. In vitro ovicidal and larvicidal potential of crude aqueous and hydro-alcoholic extracts of the bulb of O. sanctum
was investigated. Alkaloids, carbohydrates, steroids and tannins were
identified in phytochemical analyses. The various blood parameters
coupled marker enzymes and antioxidant status were also evaluated during
in vivo trial. Aqueous extract showed better EC50 and EC99
values in comparison with methanolic extract in egg hatch assay and
larval development test, respectively. However, in the larval paralysis
test, both aqueous and methanolic extracts showed almost similar
efficacy. A 77.64% reduction in fecal egg output was observed on day 14.
No deleterious ill effect was found in any of the hematological and
biochemical parameters suggesting that the plant could be safer for use
in sheep.
Keywords
- Anthelmintic activity;
- Gastrointestinal nematodes;
- India;
- Ocimum sanctum;
- Sheep
- Corresponding
author. Department of Parasitology, College of Veterinary Sciences
& Animal Husbandry, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa
Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura,
281001, India. Tel.:
+91 9045414200; fax: +91 9045414200.
Copyright © 2015 Elsevier Ltd. All rights reserved.
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