Berberine
hydrochloride attenuates lipopolysaccharide-induced endometritis in
mice by suppressing activation of NF-κB signal pathway.
Abstract
Endometritis
is a common disease in animal production and influences breeding all
over the world. Berberine is one of the main alkaloids isolated from
Rhizoma coptidis. Previous reports showed that berberine has
anti-inflammatory potential. However, there have been a limited number
of published reports on the anti-inflammatory effect of berberine
hydrochloride on LPS-induced endometritis. The purpose of the present
study was to investigate the effects of berberine hydrochloride on
LPS-induced mouse endometritis. Berberine hydrochloride was administered
intraperitoneally at 1h before and 12h after LPS induction. Then, a
biopsy was performed, and uterine myeloperoxidase (MPO) and nitric oxide
(NO) concentrations were determined. Tumor necrosis factor-α (TNF-α)
and interleukin-1β (IL-1β) levels in the uterus homogenate were measured
by ELISA. The extent of IκB-α and P65 phosphorylation was detected by
Western blot. The results showed that berberine hydrochloride
significantly attenuated neutrophil infiltration, suppressed
myeloperoxidase activity and decreased NO, TNF-αand IL-1βproduction.
Furthermore, berberine hydrochloride inhibited the phosphorylation of
the NF-κB p65 subunit and the degradation of its inhibitor, IκBα. These
findings suggest that berberine hydrochloride exerts potent
anti-inflammatory effects on LPS-induced mouse endometritis and might be
a potential therapeutic agent for endometritis.
Copyright © 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Berberine hydrochloride; Endometritis; LPS; NF-κB
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