Volume 49, Issue 10, October 2014, Pages 1757–1766
Concurrent purification and antioxidant activity of phycobiliproteins from Lyngbya sp. A09DM: An antioxidant and anti-aging potential of phycoerythrin in Caenorhabditis elegans
Highlights
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- Concurrent purification of PE, PC and APC from Lyngbya sp. A09DM.
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- A novel use of Triton X-100 in ammonium sulphate fractionation.
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- In vitro antioxidant activity of purified PE, PC and APC.
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- Phycoerythrin expands life span of Caenorhabditis elegans.
Abstract
The present study probes into the purification of phycobiliproteins, and characterization of their in vitro
anti-oxidant activity. Moreover, the study also demonstrates the use of
antioxidant virtue of phycoerythrin in moderating the phenomenon of
aging in Caenorhabditis elegans. Phycoerythrin, phycocyanin and allophycocyanin were purified successfully from Lyngbya
sp. A09DM by ammonium sulfate fractionation appended with Triton X-100
intercession. The success of protocol was examined by a series of
biochemical characterization like SDS-PAGE, native-PAGE, UV–visible
spectroscopy and fluorescence spectroscopy ensuring purity, integrity
and functionality of purified phycoerythrin, phycocyanin and
allophycocyanin. Purified phycobiliproteins were evaluated for
antioxidant and metal ion chelating activity by various in vitro
antioxidant assay systems. Results showed significant and
dose-dependent antioxidant as well as metal chelating potential of all
phycobiliproteins in decreasing order of
phycoerythrin > phycocyanin > allophycocyanin. Expansion in
lifespan and improvement in pharyngeal pumping of C. elegans were noticed upon pre-treatment with phycoerythrin (100 μg ml−1).
Moreover, phycoerythrin mediated increase in worm survival under
oxidative stress revealed that the life expansion effect of
phycoerythrin on nematode is in part by an action of its antioxidant
virtue. These results collectively added up evidence in favor of the
‘free-radical theory of aging’. The present report, for the first time,
describes antioxidant potential of phycoerythrin and its use in
extending life-span of C. elegans.
Keywords
- Phycobiliproteins;
- Lyngbya sp. A09DM;
- Triton X-100;
- Antioxidant activity, Caenorhabditis elegans;
- Anti-aging activity
Copyright © 2014 Elsevier Ltd. All rights reserved.
