Volume 55, April 2014, Pages 19–24
Inhibition of ROS production, photoprotection, and total phenolic, flavonoids and ascorbic acid content of fresh herb juice and extracts from the leaves and flowers of Tropaeolum majus
Highlights
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- Juice strongest inhibits ROS production in skin fibroblasts after UVA irradiation.
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- Hydroethanolic extract is the strongest ROS scavenger/production inhibitor in almost all used models.
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- The highest content of the polyphenols was determined in hydroethanolic extract.
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- The highest content of the ascorbic acid was determined in juice.
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- Lack of correlation between the polyphenols amount and the activity was observed.
Abstract
The
aim of our study was to determine antioxidant activity of aqueous and
hydroethanolic extracts obtained from both freeze-drying leaves and
flowers, as well as juice squeezed from the fresh herb of Tropaeolum majus L. In cell-free systems the scavenging of synthetic radical (DPPH) and two radicals (O2
−, H2O2) was evaluated. In in vitro
experiments, the effect on reactive oxygen species (ROS) production by
f-MLP-stimulated neutrophils, as well as the effect on ROS generation by
human skin fibroblasts after UV irradiation were determined. In studies
on fibroblasts, the protective effect of the extracts/juice against
cell membrane damage caused by UV irradiation was also tested. The
tested extracts/juice were chemically characterized by the determination
of the content of flavonoids, total phenols and ascorbic acid.
−, H2O2) was evaluated. In in vitro
experiments, the effect on reactive oxygen species (ROS) production by
f-MLP-stimulated neutrophils, as well as the effect on ROS generation by
human skin fibroblasts after UV irradiation were determined. In studies
on fibroblasts, the protective effect of the extracts/juice against
cell membrane damage caused by UV irradiation was also tested. The
tested extracts/juice were chemically characterized by the determination
of the content of flavonoids, total phenols and ascorbic acid.
Scavenging activity of the tested extracts/juice against synthetic radical-DPPH was low. Against H2O2 and O2− the extracts showed stronger antioxidant activity, while the juice was significantly active only against O2−.
In the human neutrophils model the hydroethanolic extract most strongly
inhibited production of ROS. The weakest activity was shown by the
juice. Studies on human skin fibroblasts showed no cytotoxic activity of
the tested extracts/juice. We also observed inhibition of ROS
production by tested extracts/juice induced by UVA as well as by UVB
irradiation. The strongest inhibition of ROS production after UVA
irradiation was shown by the juice, while after UVB irradiation the most
potent was the hydroethanolic extract. Our results partly explain the
use of extracts from nasturtium in skin diseases, such as sunburns.
− the extracts showed stronger antioxidant activity, while the juice was significantly active only against O2−.
In the human neutrophils model the hydroethanolic extract most strongly
inhibited production of ROS. The weakest activity was shown by the
juice. Studies on human skin fibroblasts showed no cytotoxic activity of
the tested extracts/juice. We also observed inhibition of ROS
production by tested extracts/juice induced by UVA as well as by UVB
irradiation. The strongest inhibition of ROS production after UVA
irradiation was shown by the juice, while after UVB irradiation the most
potent was the hydroethanolic extract. Our results partly explain the
use of extracts from nasturtium in skin diseases, such as sunburns.Graphical abstract
Keywords
- Tropaeolum majus L.;
- Tropaeolaceae;
- Reactive oxygen species;
- Neutrophils;
- Skin fibroblasts;
- Ultraviolet
Copyright © 2014 Elsevier B.V. All rights reserved.
