Full paper
Diversity of Phytophthora species newly reported from Japanese horticultural production
Highlights
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- We studied 14 Phytophthora isolates, phylogenetically and morphologically.
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- We re-identified five Phytophthora species which are new to Japan.
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- The cox1 gene is dependable for Phytophthora identification.
Abstract
Recently
developed molecular techniques facilitate the re-evaluation of
phylogenetic relationships and taxonomic classification. This study
evaluates 11 Japanese Phytophthora strains that previously had
been identified morphologically, and three additional strains that had
remained unidentified. The eleven previously identified strains
exhibited low sequence homology with the species they were assigned to
originally, but high homology with P. hedraiandra, P. multivora, P. niederhauserii, P. sansomeana, P. sp. kelmania, and P. sojae. The three previously unidentified strains showed low homology with representative strains of P. nicotianae or P. palmivora,
according to previous analysis based on rRNA gene internal transcribed
spacer (rRNA gene ITS), rRNA gene large sub-unit (LSU) and cytochrome c sub-oxidase (cox)1
sequences. To confirm previous phylogenetic identifications, we
sequenced more two genes, β-tubulin and translation elongation factor 1
alpha (EF1-α), performed phylogenetic analyses in different
gene combinations and obtained results that were in line with
morphological data. The re-identified species P. hedraiandra, P. multivora, P. niederhauserii, P. sansomeana, and P. sp. kelmania are new to Japan, adding to the Phytophthora biodiversity in this country. Taken together, this and previous phylogenetic studies indicate that cox1 is a dependable gene for Phytophthora identification.
Keywords
- Identification;
- Molecular phylogeny;
- Morphology
Copyright © 2015 The Mycological Society of Japan. Published by Elsevier Japan KK All rights reserved.
