Volume 8, March 2015, Pages 181–191
Lipidomics as a new approach for the bioprospecting of marine macroalgae — Unraveling the polar lipid and fatty acid composition of Chondrus crispus
Highlights
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- The polar lipid profile of Chondrus crispus is reported for the first time.
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- Liquid chromatography–mass spectrometry was used to characterize its lipidome.
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- Glycolipids, glycerophospholipids and betaine lipids were identified.
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- Phytyl derivatives as chlorophylls and pheophytins were also identified.
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- Molecular species bearing PUFAs (C20:4n-6, C20:5n-3, C22:6n-3) were detected.
Abstract
Marine
macroalgae, or seaweeds, have been used for direct human consumption,
as additives in the food industry, and as fertilizers in agriculture.
The fatty acid composition of several species of macroalgae has been
extensively studied, considering their nutritional value and for
phylogenetic characterization. However, the polar lipid components of
macroalgae, which include compounds with important nutraceutical and
bioactive properties, are still very poorly studied at the molecular
level.
Herein, it is reported, for the first time, a lipidomic study on the red seaweed Chondrus crispus,
namely its fatty acid and polar lipid composition through hydrophilic
interaction liquid chromatography — electrospray ionization mass
spectrometry (HILIC–ESI–MS), as an approach to better understand its
valuable properties provided from lipidic components.
The
main polar lipid groups identified include glycolipids
(sulfoquinovosyldiacylglycerols and digalactosyldiacylglycerols),
glycosphingolipids bearing ceramide backbones (galactosylceramides),
inositolphosphoceramides, glycerophospholipids (phosphatidylcholines,
lyso-phosphatidylcholines, phosphatidic acids, phosphatidylglycerols and
lyso-phosphatidylglycerols), and betaine lipids, as well as some phytyl
derivatives, as chlorophylls and pheophytins.
This
lipidomic-based approach is envisaged as very promising in the study of
algae lipid fingerprint, required to understand algae metabolism, their
dependence of environmental conditions, and also for the valorization of
macroalgae as edible products and a source of bioactive compounds.
Keywords
- Phospholipids;
- Glycolipids;
- Electrospray;
- Mass spectrometry;
- Liquid chromatography–mass spectrometry;
- Seaweed
Copyright © 2015 Elsevier B.V. All rights reserved.
