Environ Res. 2015 Sep 22. pii: S0013-9351(15)30084-0. doi: 10.1016/j.envres.2015.09.014. [Epub ahead of print]
Contribution to the risk characterization of ciguatoxins: LOAEL estimated from eight ciguatera fish poisoning events in Guadeloupe (French West Indies).
Hossen V1, Soliño L2, Leroy P1, David E3, Velge P4, Dragacci S5, Krys S1, Flores Quintana H6, Diogène J2.
Abstract
From
2010 to 2012, 35 ciguatera fish poisoning (CFP) events involving 87
individuals who consumed locally-caught fish were reported in Guadeloupe
(French West Indies). For 12 of these events, the presence of
ciguatoxins (CTXs) was indicated in meal remnants and in uncooked fish
by the mouse bioassay (MBA). Caribbean ciguatoxins (C-CTXs) were
confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)
analysis. Using a cell-based assay (CBA), and the only available
standard Pacific ciguatoxin-1 (P-CTX-1), the lowest toxins level
detected in fish samples causing CFP was 0.022µgP-CTX-1 equivalent
(eq.)·kg-1 fish. Epidemiological and consumption data were
compiled for most of the individuals afflicted, and complete data for
establishing the lowest observable adverse effects level (LOAEL) were
obtained from 8 CFP events involving 21 individuals. Based on toxin
intakes, the LOAEL was estimated at 4.2ng P-CTX-1eq./individual
corresponding to 48.4pgP-CTX-1eq.kg-1 body weight (bw).
Although based on limited data, these results are consistent with the
conclusions of the European Food Safety Authority (EFSA) opinion which
indicates that a level of 0.01µgP-CTX-1eq.kg-1 fish,
regardless of source, should not exert effects in sensitive individuals
when consuming a single meal. The calculated LOAEL is also consistent
with the U.S. Food and Drug Administration guidance levels for CTXs
(0.1µgC-CTX-1eq.kg-1 and 0.01µgP-CTX-1 eq.kg-1 fish).
Copyright © 2015 Elsevier Inc. All rights reserved.
Copyright © 2015 Elsevier Inc. All rights reserved.
