Biswas
et al.
,
IJPSR, 2011; Vol. 2(10):
2605
-
2609
ISSN: 0975
-
8232
Available online on
www.ijpsr.com
2605
IJPSR
(20
1
1
),
Vol.
2
, Issue
10
(
R
e
search
Article
)
Received
on
11
February
,
2011
; received in revised form
2
1
April
,
2011
; accepted
2
8
April
,
2011
ASSESSMENT OF CYTOTOXICITY AND ANTIBACTERIAL ACTIVITIES OF ETHANOLIC EXTRACTS OF
KALANCHOE
PINNATA
LINN. (FAMILY: C
RASSULACEAE) LEAVES AND STEMS
Subrata Kumar Biswas
*
1
, Anusua Chowdhury
1
, Joysree Das
1
, Utpal Kumar Karmakar
2
and Manik Chandra Shill
3
Department of Pharmacy, BGC Trust University
1
,
Chittagong,
Bangladesh
Life Science School, Khulna University
2
, Khuln
a, Bangladesh
IPD Pharmacy, Square Hospitals Limited
3
, Dhanmondi, Bangladesh
ABSTRACT
Objective:
In the present work, the leaves and stems of
Ka
lanchoe pinnata
Linn. (Family: Crassulaceae)
have been investigated for screening
phytochemicals, cytotoxicity and antibacterial activities.
Materials and methods:
Brine shrimp lethality (BSL) bioassay was used as a
pre
-
screening method for cytotoxic effec
t. Ethanolic extract of the plant was
tested by using this method. The agar disc diffusion method was used for
antimicrobial assay of the plant extract.
Results:
The crude extract produced the most significant cytotoxic activity
against brine shrimp
Artem
ia salina
(LC50: 100
μg/mL and LC90:
204.17μg/mL). The ethanolic extract of
Kalanchoe pinnata
Linn. showed
significant antibacterial activity against gram positive (
B. Subtilis, S. aureus)
and gram negative
(E. coli, P. aeruginosa, S. dysenteriae)
bacteria with the
zones of in
hibition ranging from 6.0±0.35 to 8.2±0.22 mm. The
phytochemical screening of the ethanolic extracts of
Kalanchoe pinnata
Linn.
showed the presence of
alkaloids, glycosides, steroids, gums, flavonoids,
saponins, reducing sugars and tannins.
Conclusion:
Th
e obtained results provide a support for the use of this plant in
traditional medicine.
The plant studied can be a potential source of
biologically active compounds as antitumor agent, antibacterial and
pesticide.
INTRODUCTION
:
Kalanchoe pinnata
Linn. (Fam
i
Assessment of Cytotoxicity and Antibacterial activities of Ethanolic Extracts of Kalanchoe pinnata Linn. (Family: Crassulaceae) leaves and stems. Available from: https://www.researchgate.net/publication/283258068_Assessment_of_Cytotoxicity_and_Antibacterial_activities_of_Ethanolic_Extracts_of_Kalanchoe_pinnata_Linn._%28Family_Crassulaceae%29_leaves_and_stems [accessed Nov 13, 2015].
