Friday, 29 January 2016

Comparative Study of Balkan Sideritis Species from Albania, Bulgaria and Macedonia

European Journal of Medicinal Plants, ISSN: 2231-0894,Vol.: 5, Issue.: 4

Original Research Article

Alban Ibraliu1, Antoaneta B. Trendafilova2, Boban D. Anđelković3, Bujar Qazimi4, Dejan M. Gođevac5, Dorina Shengjergji6, Ela Bebeci6, Gjoshe Stefkov4, Gordana Zdunic7, Ina I. Aneva8, Ina Pasho5, Jasmina Petreska-Stanoeva9, Kalina I. Alipieva2, Katarina Savikin7*, Ljuba N. Evstatieva8, Nebojsa Menkovic7, Marina I. Stefova9*, Milena Popova2, Milka B. Jadranin5, Milka N. Todorova2, Petko Denev2, Svetlana Kulevanova4, Vassya S. Bankova2*, Vilma Gurazi10 and Vilma Papajani-Toska6*
1Department of Crop Production, Faculty of Agriculture and Environment, Agricultural University of Tirana, Albania.
2Institute of Organic Chemistry with Centre of Phytochemistry, BAS, Acad. Georgi Bonchev St., bl.9, 1113 Sofia, Bulgaria.
3Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
4Institute of Pharmacognosy, Faculty of Pharmacy, Ss. Cyril and Methodius University, Vodnjanska 17,1000 Skopje, Republic of Macedonia.
5Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
6Faculty of Pharmacy, University of Medicine, Tirana, Albania.
7Institute for Medicinal Plants Research, Dr. Josif Pancic, Tadeusa Koscuska 1, 11000 Belgrade, Serbia.
8Institute of Biodiversity and Ecosystem Research BAS, 2 Gagarin St, 1113 Sofia, Bulgaria.
9Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, P.O. Box 162, 1001 Skopje, Republic of Macedonia.
10Tirana University, Faculty of Natural Sciences, Industrial Chemistry Department, Albania.


Background: Sideritis scardica and S. raeseri are very popular medicinal plants in Albania, Macedonia, and Bulgaria.
Objective: Comparison of the phenolic, flavonoid, and diterpene contents and detailed chemical profiles, as well as data on antioxidant activity, comprising plant material from different locations in the Balkan Peninsula.
Materials and Methods: Total phenolic and flavonoid contents for the studied Sideritis samples were determined spectrophotometrically. By HPLC-DAD-ESI-MSn analysis, 48 individual phenolic constituents were completely or tentatively identified. Chemical profiling of Sideritis major diterpenes and their quantification was performed by HPLC-ESI-MSD TOF analysis. A combination of two methods (ORAC and DPPH) was applied for evaluation of the antioxidant capacity of Sideritis extracts.
Results: The results obtained demonstrated that Sideritis samples (S. scardica and S. raeseri) collected from Albania, Bulgaria and Macedonia are similar in their chemical profiles (they all contain phenylethanoid glycosides, flavonoid 7-O-diglycosides and acetylated flavonoid 7-O-diglycosides, and diterpenes: main components siderol and sideridiol); and antioxidant activity. Although some differences exist, they are not essential.
Conclusion: This fact is a good basis for the formulation of range for parameters for quality control of Sideritis extracts and plant material. The studied cultivated plant material is similar in its chemical composition, profile and antioxidant activity to the wild growing samples.

Keywords :
Sideritis; chemical profiling; phenolics; flavonoids; diterpenes.

DOI : 10.9734/EJMP/2015/14389