Volume 38, Issues 8–9, October–November 2005, Pages 1079–1085
Third International Congress on Pigments in Food
Third International Congress on Pigments in FoodAbstract
Two cultivars (Thiessen and Smoky) of Saskatoon berries (Amelanchier alnifolia Nutt.) possess free radical scavenging activities in a concentration-dependent manner, whereas Thiessen presented higher (p < 0.05)
activity compared to Smoky due to its relatively abundant anthocyanin
content. Total anthocyanin content significantly corresponded to free
radical scavenging activities (p < 0.05). Solvent
fractionation revealed that the free radical scavenging components,
i.e., anthocyanin, predominantly occurred in the ethyl acetate and n-butanol
extracted fractions, suggesting that active components are more likely
to occur in glycoside forms. HPLC further confirmed the existence of
cyanidin-3-O-galactoside and cyanidin-3-O-glucoside as
leading anthocyanins in the Saskatoon berries. Furthermore, Saskatoon
berries extracts from both cultivars suppressed peroxy-radical induced
intracellular oxidation in a concentration-dependent manner without
affecting cell viability. The obtained results provided useful
information for the future application of Saskatoon berries in the
nutraceutical industry.
Keywords
- Saskatoon berry (Amelanchier alnifolia Nutt.);
- Anthocyanin;
- Free radical;
- Intracellular oxidation
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