Volume 50, October 2013, Pages 419–427
Highlights
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- Twenty two Rubus cultivars and wild species of Bulgarian collection are analyzed.
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- Phenolic acids and flavonoides in the leaves are determined by HPLC-UV.
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- Hierarchical clustering and principal component analyses of data are presented.
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- Caffeic acid is the dominant phenolic compound responsible for the clustering.
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- Bulgarian raspberry elite clones have the highest amounts of phenolic compounds.
Abstract
Leaves of 22 accessions of the Bulgarian raspberry (Rubus L.) germplasm collection – five Bulgarian and seven foreign cultivars, eight elite clones and two wild species, Rubus occidentalis L. and Rubus odoratus L., were analysed for phenolic constituents. The quantitative determination of caffeic (1), p-coumaric (2) and ferulic (3) acids, hyperoside (4), tiliroside (5), and isoquercitrin (6)
was performed by RP-HPLC using linear gradient elution and UV detection
at 254 and 310 nm. The detection limits ranged from 0.23 μg/ml (4) to 0.55 μg/ml (2).
Caffeic acid was the dominant phenolic acid in the majority of the
samples being present in amounts between 0.05 ± 0.01 mg/g dry weight in R. occidentalis
and 1.43 ± 0.06 mg/g in the cultivars. The highest content of flavonols
was found in the Bulgarian raspberry elite clones: 1.70 ± 0.002 mg/g (6), 0.60 ± 0.004 mg/g (5) and 0.97 ± 0.01 mg/g (4).
Data were analyzed by hierarchical clustering (HC) and principal
component analysis (PCA). The HC and PCA scoring plot showed that the
samples could be classified into three clusters. Cluster C1 comprised
cultivars characterized by high content of phenolic acids (1–3). Seven cultivars and the wild species R. occidentalis
formed the cluster C2 presenting low content of phenolic compounds.
Cluster C3 grouped the Bulgarian raspberry elite clones and R. odoratus sharing the highest content of flavonols (5 and 6). The Bulgarian elite clone E23617 displayed the highest content of the studied phenolic derivatives. The PCA loading plot showed that 1 can be used to distinguish between different raspberry varieties.
Keywords
- Raspberry;
- Phenolic acids;
- Flavonoids;
- Caffeic acid;
- PCA;
- Hierarchical clustering
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