a
Key Laboratory of Environment Correlative Dietology, Ministry of
Education, College of Food Science and Technology, Huazhong Agricultural
University, Wuhan, Hubei, China
b
Georgetown University Medical Center, Washington, DC, United States
Abstract
Antifungal
components, from poplar buds active fraction (PBAF) against Penicillium
italicum, the causal agent of blue mold in citrus fruits, were
identified and possible action modes were investigated. Pinocembrin,
chrysin and galangin were determined as active components in PBAF, using
HPLC and HPLC-MS analysis. The antifungal activity is stable at
temperatures ranging from 4°C to 100°C and pH levels ranging from 4 to
8. In the presence of PBAF, the hyphae become shriveled, wrinkled and
the cell membrane became seriously disrupted. Further investigation on
cell permeability, nucleic acid content and alkaline phosphatase suggest
that the cell membrane might be the target. Mycelial oxygen consumption
and the respiration-related enzymatic activity of succinate
dehydrogenase, malate dehydrogenase and ATPase were all inhibited by
PBAF. We propose that PBAF is a potentially useful alternative for blue
mold control and may act against P. italicum by interfering with
respiration and disrupting the cell membrane. © 2015 Elsevier Ltd. All
rights reserved.
Author keywords
Active constituents; Mode of action; P. italicum; Poplar buds; Respiration
Indexed keywords
Engineering controlled terms: Alkalinity; Antifungal agents; Cells; Citrus fruits; Enzyme activity; Flavonoids; Molds; Nucleic acids; Phosphatases
Active constituents; Mode of action; P. italicum; Poplar buds; Respiration
Engineering main heading: Cytology
EMTREE drug terms: adenosine triphosphatase; alkaline
phosphatase; chrysin; galangin; malate dehydrogenase; nucleic acid;
oxygen; pinocembrine; succinate dehydrogenase
EMTREE medical terms: antifungal activity; Article;
bud; cell membrane; cell membrane permeability; cell respiration; citrus
fruit; concentration (parameters); controlled study; electric
conductivity; enzyme activity; fungal cell; fungus hyphae; high
performance liquid chromatography; mass spectrometry; mycelial growth;
mycelium; nonhuman; oxygen consumption; Penicillium italicum; pH;
Populus; spore germination; temperature
Chemicals and CAS Registry Numbers: adenosine
triphosphatase, 37289-25-1, 9000-83-3; alkaline phosphatase, 9001-78-9;
chrysin, 12624-02-1, 480-40-0; galangin, 548-83-4; malate dehydrogenase,
9001-64-3; oxygen, 7782-44-7; pinocembrine, 480-39-7; succinate
dehydrogenase, 9002-02-2, 9028-10-8
