(Article)
a Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB/CGB-UTAD), University of Trás-os-Montes e Alto Douro, Vila Real, Portugal
b Department of Botany, University of Porto, Porto, Portugal
c Department of Genetics and Biotechnology, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal
b Department of Botany, University of Porto, Porto, Portugal
c Department of Genetics and Biotechnology, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal
Abstract
Iberian Buxus spp. are represented by B. sempervirens, restricted to the Cantabrian, eastern Iberian Peninsula, and northeastern mountain systems, emerging scarcely in Portugal, and by B. balearica that occurs in the Betic deep river valleys and in the Balearic islands. The genetic affinity existent between fifteen populations of B. sempervirens was evaluated by seven ISSRs primers. Moreover, leaf morphoanatomical measurements indicative of leaf performance (such as leaf area, leaf mass per unit area, width, length, shape factor, tissues thickness, stomatal density, and quantification of epi- and intracuticular waxes) and environmental characterization were conducted to get insight into the functional ecology of the genus Buxus. Six populations of B. balearica and 15 of B. sempervirens, collected in the Iberian Peninsula and the Balearic islands, were included to establish the interspecific ranges. A functional ecological description by morpho-anatomical analysis of leaves and a genetic and environmental approach exposed differences between Pyrenaean–Cantabrian–Portuguese and Iberian B. sempervirens populations. Genetic relationships among Buxus populations were investigated using intersimple sequence repeat (ISSR) markers. Seven ISSR primers generated a total of 159 unambiguous and repeatable bands, of which 156 (98.1%) were polymorphic. These divergences for Iberian B. sempervirens are explained by the isolation in their distributions since the Oligocene. Previsions for future climate change scenarios confirm those biogeographic divergences for the Iberian B. sempervirens. © TÜBİTAK.
Author keywords
B. sempervirens; Climatic characterization; Genetic flows; Leaf anatomy; Molecular marker; Oligocene–Holocene evolution
Indexed keywords
GEOBASE Subject Index: anatomy; angiosperm; biogeography; divergence; flora; gene flow; leaf morphology; Oligocene
Regional Index: Balearic Islands; Iberian Peninsula; Mediterranean Sea; Portugal; Spain
Species Index: Buxus; Buxus balearica; Buxus sempervirens
ISSN: 1300008XSource Type: Journal Original language: English
DOI: 10.3906/bot-1409-7Document Type: Article
Publisher: Turkiye Klinikleri Journal of Medical Sciences
