| 1815 | Otto Von Bismarck, chancellor of Germany. | |
| 1868 | Edmond Rostand, French dramatist (Cyrano de Bergerac). | |
| 1929 | Milan Kundera, Czech writer (The Farewell Party, The Unbearable Lightness of Being). |
Volume 110, April 2016, Pages 189–195
Rational quality assessment procedure for less-investigated herbal medicines: Case of a Congolese antimalarial drug with an analytical report 1
- a Institute of Organic Chemistry, University of Würzburg, Am Hubland, 97074 Würzburg, Germany
- b Faculty of Pharmaceutical Sciences, University of Kinshasa, B.P. 212, Kinshasa XI, Congo
- Received 31 January 2016, Revised 9 March 2016, Accepted 12 March 2016, Available online 20 March 2016
Abstract
Herbal
medicines are the most globally used type of medical drugs. Their high
cultural acceptability is due to the experienced safety and efficiency
over centuries of use. Many of them are still phytochemically
less-investigated, and are used without standardization or quality
control. Choosing SIROP KILMA, an authorized Congolese antimalarial
phytomedicine, as a model case, our study describes an interdisciplinary
approach for a rational quality assessment of herbal drugs in general.
It combines an authentication step of the herbal remedy prior to any
fingerprinting, the isolation of the major constituents, the development
and validation of an HPLC-DAD analytical method with internal markers,
and the application of the method to several batches of the herbal
medicine (here KILMA) thus permitting the establishment of a
quantitative fingerprint. From the constitutive plants of KILMA,
acteoside, isoacteoside, stachannin A, and pectolinarigenin-7-O-glucoside
were isolated, and acteoside was used as the prime marker for the
validation of an analytical method. This study contributes to the
efforts of the WHO for the establishment of standards enabling the
analytical evaluation of herbal materials. Moreover, the paper describes
the first phytochemical and analytical report on a marketed Congolese
phytomedicine.
Graphical abstract
Abbreviations
- INERA, Institut National pour l'Etude et la Recherche Agronomiques;
- HPLC, high performance liquid chromatography;
- DAD, diode array detector;
- CRS, chemical reference substance;
- ICH, International Conference on Harmonization;
- ANOVA, analysis of variance;
- SD, standard deviation;
- RSD, repeatability standard deviation;
- NMR, nuclear magnetic resonance;
- COSY, correlation spectroscopy;
- TOCSY, total correlation spectroscopy;
- HSQC, heteronuclear single quantum correlation;
- HMBC, heteronuclear multiple bond correlation;
- NOESY, nuclear overhauser effect spectroscopy;
- HR-ESI-MS, high resolution-electrospray ionization-mass spectrometry
Keywords
- Herbal medicine;
- Fingerprinting;
- Authentication;
- Validation;
- Acteoside;
- SIROP KILMA
Chemical compounds studied in this article
- Acteoside (PubChem CID: 5,281,800);
- Stachannin A (PubChem CID: 44,258,461);
- Pectolinarigenin-7-O-glucoside (PubChem CID: 44,258,439);
- Isoacteoside (PubChem CID: 6,476,333)
Copyright © 2016 Elsevier B.V. All rights reserved.
