Polyphenols, antioxidants, and antimutagenic effects of Copaifera langsdorffii fruit

Food Chemistry

Volume 197, Part B, 15 April 2016, Pages 1153–1159

• Ângela Giovana Batista^a,
• Angélica Simões Ferrari^b,
• Débora Cristina da Cunha^b,
• Juliana Kelly da Silva^a,
• Cinthia Baú Betim Cazarin^a,
• Luiz Claudio Correa^d,
• Marcelo Alexandre Prado^a,
• Luciano Bruno de Carvalho-Silva^b,
• Elizabethe Adriana Esteves^c,
• Mário Roberto Maróstica Júnior^{a, ,}

• ^a School of Food Engineering, University of Campinas (UNICAMP), P.O. Box 6121, 13083-862 Campinas-SP, Brazil
• ^b School of Nutrition, Federal University of Alfenas, MG (UNIFAL-MG), Brazil
• ^c Nutrition Department, Federal University of Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Minas Gerais, Brazil
• ^d Brazilian Agricultural Research Corporation, Embrapa Tropical Semi-arid, Petrolina, Pernambuco 56302-970, Brazil

Received 17 April 2015, Revised 21 October 2015, Accepted 17 November 2015, Available online 18 November 2015

 

doi:10.1016/j.foodchem.2015.11.093

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Highlights

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Copaiba fruit showed high polyphenol contents and antioxidant capacity.

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Copaiba fruit pulp is a source of gallic acid.

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Acid hydrolysis improved quantification of catechin and epicatechin in copaiba fruit.

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100 mg copaiba fruit pulp kg⁻¹ showed antimutagenic activity.

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Abstract

Copaifera langsdorffii (copaiba) is a Brazilian exotic fruit, poorly studied regarding its bioactive composition. The aim of this study was to determine bioactive compounds, antioxidant and antimutagenic activities of copaiba pulp. The samples were extracted with different solvents in order to analyze polyphenol compounds (Folin Ciocalteau and HPLC–DAD), total flavonoids (reaction with AlCl₃) and antioxidant capacity (FRAP, ORAC, DPPH). The copaiba fruit showed high polyphenol content and antioxidant capacity. Phenolic compounds, such as gallic acid, epicatechin gallate, catechin, epicatechin and isoquercitrin, were identified in the copaiba pulp. Despite the antioxidant capacity, the highest dose of copaiba showed no antimutagenic effects in the in vivo study. The dose which showed antimutagenic activity was 100 mg kg⁻¹.

Keywords

• Copaiba;
• Gallic acid;
• Micronuclei;
• Antioxidant capacity

Chemical compounds studied in this article

• Gallic acid (PubChem CID: 370);
• (−)-Epicatechin (PubChem CID: 72276);
• (+)-Catechin (PubChem CID: 964);
• (−)-Epicatechin gallate (PubChem CID: 367141);
• Isoquercitrin (PubChem CID: 5280804)

Corresponding author at: Departamento de Alimentos e Nutrição, Rua Monteiro Lobato, 80, Cidade Universitária, Campinas-SP, Brazil.

Copyright © 2015 Published by Elsevier Ltd.