The new flavonoid gnaphaloside C, which was identified based on UV, MS, and NMR spectral data as spinacetin-7-O-(6″-O-caffeoyl)-β-D-glucopyranoside, and 24 known compounds including for the first time from this species quercetagetrin, quercetagetin-7-O-(6″-O-caffeoyl)glucoside, patulitrin, tinctoside, and spinacetin-7-O-glucoside were isolated from the aerial part of Gnaphalium uliginosum. HPLC determined that G. uliginosum contained mainly caffeoylquinic acids (10.54–48.48 mg/g). The flavonoid content was 1.20–16.55 mg/g.