2011 Isolation of a novel catechin from Bergenia rhizomes that has pronounced lipase-inhibiting and antioxidative properties

Fitoterapia. 2011 Mar;82(2):212-8. doi: 10.1016/j.fitote.2010.09.013. Epub 2010 Oct 12.

Ivanov SA1, Nomura K, Malfanov IL, Sklyar IV, Ptitsyn LR.

Author information

• 1Ajinomoto-Genetika Research Institute, 1-st Dorozhny pr. 1-1, Moscow 117545, Russia.

Abstract

An aqueous ethanol extract of Bergenia crassifolia rhizomes strongly inhibited human pancreatic lipase activity and increased scavenging of DPPH free radicals in vitro. Chromatographic separation of this extract led to isolation of the hydrolysable tannins (+)-catechin 3,5-di-O-gallate (1) and (+)-catechin 3-O-gallate (2). This is the first report of the isolation of compound 1 from plant material. This compound strongly inhibited human pancreatic lipase (with an IC(50) value of 0.42 μg/ml) and exhibited a remarkable free radical-scavenging ability (with an SC(50) value of 1.04 μg/ml). The chemical structures of 1 and 2 were elucidated using MS, NMR and chemical approaches.