Volume 51, Issue 6, 2015, Pages 368–377
Original Research Article
- Open Access funded by The Lithuanian University of Health Sciences
- Under a Creative Commons license
Abstract
Background and objective
The
purpose of the present study was to examine the anti-arthritic and
antioxidant effects of herbal and active organic ingredient complex (EM
1201) in rats with experimental adjuvant arthritis (AA).
Materials and methods
AA
was induced in 30 male Wistar rats by intradermal injection of complete
Freund's adjuvant into the left hind paw. The course of disease in 30
rats in response to the treatment with EM 1201 and diclofenac, the
parameters including body weight, joint swelling, blood indices
pro-/antioxidant status of blood serum, and histology of joints and the
liver, were investigated.
Results
Preparation
EM 1201 showed anti-inflammatory effect analogous to diclofenac,
improved blood indices, significantly decreased joint swelling and
histological changes in them. Joint swelling was suppressed by 29%–42.8%
and 9.3%–34.4% in response to administration of EM 1201 and diclofenac
during the entire experiment. Both preparations significantly suppressed
pannus formation, general inflammatory reaction and edema in soft
periarticular tissues and synovium, diminished MDA level and elevated
AOA in the blood serum. Significantly lower absolute and relative weight
of the liver and lower dystrophic processes in it, and general
inflammatory infiltration of hepatic stroma proved the positive effect
of treatment with EM 1201.
Conclusions
The
present study suggests that EM 1201 has protective activity against
arthritis and demonstrated its potential beneficiary effect analogical
to diclofenac. Anti-inflammatory and anti-oxidative effect of EM 1201 in
rats with AA support the need of further investigations by using it as
supplementary agent alone or together with other anti-arthritic drugs in
the treatment of rheumatoid arthritis.
Keywords
- Herbal preparation EM 1201;
- Adjuvant arthritis;
- Diclofenac