a
Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, Poznań, Poland
b INCELL Corporation, LLC, San Antonio, TX, United States
b INCELL Corporation, LLC, San Antonio, TX, United States
Abstract
Elderberry (EDB) Sambucus nigra L. is one of the oldest medicinal plants
which is useful for therapeutic and nutritional purposes due to a large
amount of biologically active constituents, including compounds with a
high antioxidant capacity. The present study focused on the antioxidant
potential of the colon-available EDB fruit extract, derived from the
artificial gastrointestinal tract, with regard to human colonic mucosa
cells cultured in vitro. Despite the significant loss of EDB bioactive
compounds due to the digestion process, the colon-digested extract was
able to reduce the excessive intracellular ROS production (22%) and
oxidative DNA damage (46%) in the colon cells at a dose of 1 mg of
freeze-dried EDB powder/ml. Moreover, the colon-digested EDB extract
inhibited oxidant-induced mutagenicity (26%) in the Salmonella
typhimurium TA102 strain, as determined by the Ames test. In conclusion,
the current in vitro study confirmed that the fruits of S. nigra are
capable of protecting colonic cells against the detrimental effects of
oxidative stress. © 2015 Elsevier Ltd. All rights reserved.
Author keywords
Antimutagenic activity; Antioxidant capacity; Colon cells; DNA damage; Elderberry; ROS generation
Indexed keywords
Engineering controlled terms: Antioxidants; Cells; Fruits; Salmonella
Antimutagenic activity; Antioxidant capacity; Colon cells; DNA damages; Elderberry; Ros generations
Engineering main heading: Cytology
EMTREE drug terms: 4 hydroxybenzoic acid; anthocyanin;
cyanidin 3 glucoside; cyanidin 3 o rutinoside; cyanidin 3 o
sambubioside; cyanidin 3 o sambubiosyl 5 o glucoside; cyanidin 3,5 o
diglucoside; gallic acid; pelargonidin 3 o glucoside; pelargonidin 3 o
sambubioside; peonidin 3 o glucoside; peonidin 3 o sambubioside;
quercetin; quinic acid; reactive oxygen metabolite; Sambucus nigra
extract; unclassified drug
EMTREE medical terms: aerobic metabolism; antioxidant
activity; Article; colon mucosa; cytotoxicity; digestion; electrospray
mass spectrometry; fruit; genotoxicity; high performance liquid
chromatography; human; human cell; in vitro study; mutagenic activity;
mutagenicity; nonhuman; oxidative stress; Salmonella enterica serovar
Typhimurium
Chemicals and CAS Registry Numbers: 4 hydroxybenzoic
acid, 456-23-5, 99-96-7; cyanidin 3 glucoside, 7084-24-4; gallic acid,
149-91-7; quercetin, 117-39-5; quinic acid, 36413-60-2, 562-73-2,
77-95-2, 7729-33-1
ISSN: 03088146
CODEN: FOCHDSource Type: Journal
Original language: English
DOI: 10.1016/j.foodchem.2015.11.017Document Type: Article
Publisher: Elsevier Ltd
