Antimycobacterial triterpenes from the Canadian medicinal plant Sarracenia purpurea

Journal of Ethnopharmacology

Volume 188, 21 July 2016, Pages 200-203

  (Article)

Morrison, S.A.^a, 

Li, H.^a, 

Webster, D.^b, 

Johnson, J.A.^a, 

Gray, C.A.^ac 

 

^a  Department of Biological Sciences, University of New Brunswick, Saint John, New Brunswick, Canada
^b  Division of Infectious Diseases, Department of Medicine, Saint John Regional Hospital, Saint John, New Brunswick, Canada
^c  Department of Chemistry, University of New Brunswick, Saint John, New Brunswick, Canada 

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Abstract

Ethnopharmacological relevance The purple pitcher plant, Sarracenia purpurea, is a medicinal plant used by the Canadian First Nations to treat a wide variety of illnesses. The Mi'kmaq and Wolastoqiyik (Maliseet) peoples of Eastern Canada have traditionally used infusions of S. purpurea for the treatment of tuberculosis-like symptoms. Previous investigations have shown methanolic extracts of S. purpurea to possess antimycobacterial activity. Aim of the study To isolate and identify antimycobacterial constituents from S. purpurea. Materials and Methods Methanolic extracts of S. purpurea were subjected to bioassay guided fractionation using the microplate resazurin assay (MRA) to assess inhibitory activity against Mycobacterium tuberculosis strain H37Ra. The antimycobacterial constituents were identified by NMR, MS and polarimetry. Results The triterpenes betulinaldehyde, β-sitosterol, betulinic acid, and ursolic acid were isolated from S. purpurea. Betulinaldehyde, betulinic acid, and ursolic acid exhibited MICs of 450, 950, and 450 μM and IC₅₀s of 98, 169, and 93 μM against M. tuberculosis H37Ra respectively whilst β-sitosterol was inactive (MIC and IC₅₀ of >1000 μM). Conclusions Betulinaldehyde, betulinic acid, and ursolic acid were identified as the principal constituents responsible for the antimycobacterial activity of S. purpurea. This work is consistent with the ethnopharmacological use of S. purpurea by Canadian First Nations as a treatment against infectious diseases. © 2016 Elsevier Ireland Ltd. All rights reserved.

Author keywords

Antimycobacterial; Betulinaldehyde; Betulinic acid; Mycobacterium tuberculosis; Sarracenia purpurea; Ursolic acid; β-Sitosterol

ISSN: 03788741 CODEN: JOETDSource Type: Journal Original language: English

DOI: 10.1016/j.jep.2016.04.052Document Type: Article

Publisher: Elsevier Ireland Ltd

Funding Details

Acronym; Sponsor: NBIF; New Brunswick Innovation Foundation
Number; Acronym; Sponsor: 350764-2009; NSERC; New Brunswick Innovation Foundation

References (19)

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  Gray, C.A.; Department of Biological Sciences, University of New Brunswick, Canada; email:cgray@unb.ca
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