Anti-inflammatory effect of natural and semi-synthetic phthalides

Instituto de Química de la Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, Coyoacán, 04510 México, D.F, México

Received 22 September 2014, Revised 13 January 2015, Accepted 15 January 2015, Available online 23 January 2015

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doi:10.1016/j.ejphar.2015.01.026

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Abstract

This study evaluated the potential anti-inflammatory effects of natural phthalides, isolated from Ligusticum porteri, and of semi-synthetic phthalides. Anti-inflammatory activity was investigated in two mouse models; one with ear edema, induced with 12-O-tetradecanoylphorbol-13-acetate, and the other with paw edema, induced with carrageenan. The effect on the RAW 264.7 stimulated with lipopolysaccharide cells was evaluated and after application of 12-O-tetradecanoylphorbol-13-acetate, the activity of myeloperoxidase was assessed to serve as an index of leukocytes infiltration together with the histological evaluations. We also assessed the inhibition of cyclooxygenases 1 and 2 in vitro. Our results demonstrated that administration of semi-synthetic phthalides significantly inhibited the ear edema induced by 12-O-tetradecanoylphorbol-13-acetate, and reduced the paw edema caused by carrageenan. The anti-inflammatory activity of phthalides could, in part, be explained by the reduction in myeloperoxidase activity and the infiltration of leukocytes. The semi-synthetic phthalides also inhibited the production of oxide nitric in RAW cells.

Chemical compounds studied in this article

Z-ligustilide (PubChem CID: 529865);
diligustilide (PubChem CID: 70698035);
tokinolide B (PubChem CID: 70681959)

Keywords

Inflammation;
Semi-synthetic phthalides;
12-O-tetradecanoylphorbol-13-acetate;
Carrageenan;
Myeloperoxidase;
Oxide nitric synthase

: Corresponding author. Tel.: +52 55 56 22 44 45; fax: +52 55 56 16 22 17.

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Saturday, 15 August 2015