World Applied Sciences Journal 30 (11): 1599-1604, 2014 ISSN 1818-4952 © IDOSI Publications, 2014 DOI: 10.5829/idosi.wasj.2014.30.11.14220 Corresponding Author: Nina Georgievna Machavariani, Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, Bolshaya Pirogovskaya Street, 11, 119021, Moscow, Russian Federation 1599 Isolation of Endophytic Actinomycetes from Medicinal Plants of the Moscow Region, Russia Nina Georgievna Machavariani, Tatyana Dmitrievna Ivankova, Olga Nikolaevna Sineva and Larissa Petrovna Terekhova Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, Bolshaya Pirogovskaya Street, 11, 119021, Moscow, Russian Federation Abstract: Endophytic bacteria are microorganisms that colonize the internal tissues of the plant without causing any external sign of infection or negative effect on their host. In the present study, endophytic actinomycetes were isolated from leaves of medicinal plants collected in Moscow and Moscow region. To isolate actinomycetes we used a method developed by us on the basis of known techniques. The main modification consisted in the pretreatment of leaves with solutions of biologically active substances of plant origin - heteroauxin and zircon. Using the modification method of this study helps to increase numbers of rare actinomycetes isolated from the medicinal plants, which are potentially unique source of novel bioactive metabolites for medicinal and biotechnological practice.

World Applied Sciences Journal 30 (11): 1599-1604, 2014 ISSN 1818-4952 © IDOSI Publications, 2014 DOI: 10.5829/idosi.wasj.2014.30.11.14220


Corresponding Author: Nina Georgievna Machavariani, Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, Bolshaya Pirogovskaya Street, 11, 119021, Moscow, Russian Federation 1599


Isolation of Endophytic Actinomycetes from Medicinal Plants of the Moscow Region, Russia Nina Georgievna Machavariani, Tatyana Dmitrievna Ivankova, Olga Nikolaevna Sineva and Larissa Petrovna Terekhova Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, Bolshaya Pirogovskaya Street, 11, 119021, Moscow, Russian Federation


Abstract: Endophytic bacteria are microorganisms that colonize the internal tissues of the plant without causing any external sign of infection or negative effect on their host. In the present study, endophytic actinomycetes were isolated from leaves of medicinal plants collected in Moscow and Moscow region. To isolate actinomycetes we used a method developed by us on the basis of known techniques. The main modification consisted in the pretreatment of leaves with solutions of biologically active substances of plant origin - heteroauxin and zircon. Using the modification method of this study helps to increase numbers of rare actinomycetes isolated from the medicinal plants, which are potentially unique source of novel bioactive metabolites for medicinal and biotechnological practice.