Volume 131, Issue 3, 1 April 2012, Pages 761-767
Effect of in vitro digestion upon the antioxidant capacity of aqueous extracts of Agrimonia eupatoria, Rubus idaeus, Salvia sp. and Satureja montana (Article)
a
CBQF/Escola Superior de Biotecnologia, Universidade Católica
Portuguesa, Rua Dr. António Bernardino de Almeida, P-4200-072 Porto,
Portugal
b Department of Biochemistry (U38-FCT), Faculty of Medicine, University of Porto, Alameda Prof. Hernâni Monteiro, P-4200-319 Porto, Portugal
c Department of Chemistry, Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, P-4169-007 Porto, Portugal
b Department of Biochemistry (U38-FCT), Faculty of Medicine, University of Porto, Alameda Prof. Hernâni Monteiro, P-4200-319 Porto, Portugal
c Department of Chemistry, Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, P-4169-007 Porto, Portugal
Abstract
An in vitro
digestion/Caco-2 cell culture model was used to assess the stability and
bioavailability of phenolic compounds in aqueous extracts of four herbal infusions, traditionally used as functional drinks in Portugal. Alterations in antioxidant power were monitored by ABTS+,
whereas the profile of phenolic compounds was ascertained by HPLC-DAD.
The bioavailability of rutin - an important flavonoid present in such
extracts, and thus a representative of those compounds, was assessed
across Caco-2 cells via LC-MS/MS. Our results indicate that several
antioxidant compounds are not affected by the (simulated) digestive
process, whereas others undergo decreases in their activity throughout
said process; a few have their antioxidant capacity hampered under
stomach-like conditions. It was also found that rutin can be transported
across the Caco-2 cell barrier. © 2011 Elsevier Ltd. All rights
reserved.
Author keywords
Agrimony; Antioxidant profile; Caco-2 cells; Raspberry; Sage; Savory
Indexed keywords
Agrimony; Antioxidant profile; Caco-2 cells; Raspberry; Sage; Savory
Engineering controlled terms: Alcohols; Beverages; Biochemistry; Cell culture; Cells; Phenols
Engineering main heading: Solvent extraction
EMTREE drug terms: Agrimonia eupatoria extract; phenol
derivative; plant extract; Rubus idaevus extract; rutoside; Salvia
extract; Satureja montana extract; unclassified drug
EMTREE medical terms: Agrimonia; agrimonia eupatoria;
antioxidant activity; article; cell strain CACO 2; controlled study;
digestion; drug bioavailability; drug stability; drug transport; high
performance liquid chromatography; in vitro study; liquid
chromatography; mass spectrometry; rubus idaeus; Salvia; satureja
montana; simulation
Species Index: Agrimonia; Agrimonia eupatoria; Rubus glaucus; Rubus idaeus; Salvia; Satureja montana
Chemicals and CAS Registry Numbers: rutoside, 153-18-4, 22519-99-9
ISSN: 03088146
CODEN: FOCHDSource Type: Journal
Original language: English
DOI: 10.1016/j.foodchem.2011.09.030Document Type: Article
