Monday, 26 October 2015

Plantago maxima leaves extract inhibits adipogenic action of a high-fat diet in female Wistar rats

Volume 53, Issue 3, 2014, Pages 831-842

Department of Biochemistry, Orenburg State Medical Academy, Sovetskaya St., 6, 460014 Orenburg, Russian Federation
Department of Pathologic Anatomy, Orenburg State Medical Academy, Sovetskaya St., 6, 460014 Orenburg, Russian Federation
Laboratory of Cellular Symbiosis, Institute of Cellular and Intracellular Symbiosis, Ural Branch of Russian Academy of Sciences, Pionerskaya St., 11, 460000 Orenburg, Russian Federation


Purpose: The primary objective of this study is to investigate the content of biologically active compounds producing an antioxidant effect in Plantago maxima and their influence on main mechanisms of dietary obesity development. Methods: Biologically active compounds in P. maxima were tested using paper chromatography. In in vivo experiment, high-fat-fed Wistar rats obtained P. maxima water extract for 3 months. Morphometric parameters, weight gain, serum adipokines, and cytokines, as well as oxidative stress biomarkers in rats' tissues were evaluated. Gut microflora was also examined. Results: Plantago maxima leaves used in the experiment contained significant amount of flavonoids, iridoids, phenol carboxylic acids, and tannins and ascorbic acid. Our in vivo experiment data demonstrate that P. maxima water extract prevents excessive adiposity in a diet-induced model. P. maxima consumption reduced serum leptin (twofold), macrophage chemoattractant protein-1 (sevenfold), tumor-necrosis factor-α (25 %), and interleukine-6 (26 %) levels. P. maxima water extract decreased adipose tissue oxidative stress biomarkers in rats fed a high-fat diet. In addition, increased bacterial growth in the diet-induced obesity model was reversed by the P. maxima extract treatment. Conclusion: Plantago maxima water extract possessed antiadipogenic, antidiabetic, antiinflammatory, antioxidant activity, and normalized gut microflora in a rat model of diet-induced excessive adiposity due to a high content of biologically active compounds. © 2013 Springer-Verlag Berlin Heidelberg.

Author keywords

Endocrine dysfunction; Excessive adiposity; Inflammation; Oxidative stress; Phytochemicals; Plantago maxima

Indexed keywords

EMTREE drug terms: apigenin; ascorbic acid; biological marker; carboxylic acid; flavonoid; interleukin 6; iridoid; leptin; luteolin; luteolin 7 glucoside; macrophage chemoattractant protein 1; membrane protein; phenol; plant extract; Plantago maxima extract; tannin derivative; tumor necrosis factor alpha; unclassified drug; water; adipocytokine; antiobesity agent; antioxidant; biological marker; cytokine; plant extract
EMTREE medical terms: adipose tissue; animal experiment; animal model; animal tissue; antidiabetic activity; antiinflammatory activity; antioxidant activity; article; bacterial growth; controlled study; female; in vivo study; intestine flora; lipid diet; medicinal plant; nonhuman; obesity; oxidative stress; paper chromatography; plant leaf; Plantago; Plantago maxima; rat; weight gain; adipogenesis; animal; bacterial count; blood; chemistry; colon; diet supplementation; Gram negative bacterium; growth, development and aging; intraabdominal fat; isolation and purification; jejunum; metabolism; microbiology; obesity; pathology; Plantago; Russian Federation; Wistar rat
MeSH: Adipogenesis; Adipokines; Adiposity; Animals; Anti-Obesity Agents; Antioxidants; Biological Markers; Colon; Colony Count, Microbial; Cytokines; Dietary Supplements; Female; Gram-Negative Bacteria; Intra-Abdominal Fat; Jejunum; Obesity; Oxidative Stress; Plant Extracts; Plant Leaves; Plantago; Rats, Wistar; Russia
Medline is the source for the MeSH terms of this document.
Chemicals and CAS Registry Numbers: apigenin, 520-36-5; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; luteolin, 491-70-3; luteolin 7 glucoside, 5373-11-5; phenol, 108-95-2, 3229-70-7; water, 7732-18-5;Adipokines; Anti-Obesity Agents; Antioxidants; Biological Markers; Cytokines; Plant Extracts
ISSN: 14366207 CODEN: EJNUFSource Type: Journal Original language: English
DOI: 10.1007/s00394-013-0587-6 PubMed ID: 24077693Document Type: Article
Publisher: Dr. Dietrich Steinkopff Verlag GmbH and Co. KG