Volume 5, Issue 2, February 2015, Pages 136–150
Document heading
Monograph: In vitro efficacy of 30 ethnomedicinal plants used by Indian aborigines against 6 multidrug resistant Gram-positive pathogenic bacteria
Abstract
Objective
To monitor in vitro
antibacterial activities of leaf extracts of 30 common and non-common
plants used by aborigines in Kalahandi district, Odisha, against 6
clinically isolated multidrug resistant (MDR) Gram-positive bacteria of 3
genera, Staphylococcus, Streptococcus, and Enterococcus.
Methods
The
antibiotic sensitivity patterns of 6 bacterial strains were studied
with the disk-diffusion method with 17 antibiotics belonging to 8
classes. Monitored plants have ethno-medicinal use and several are used
as traditional medicines. Antibacterial properties were studied with the
agar-well diffusion method. Minimum inhibitory concentration and
minimum bactericidal concentration values of ethanolic and aqueous
extracts of plants were determined by the microbroth-dilution method.
Results
Ethanolic
plant-extracts had the better antibacterial potencies in comparison to
their corresponding aqueous extracts. Plants with most conspicuous
antibacterial properties in controlling MDR strains of Gram-positive
bacteria were aqueous and ethanolic extracts of plants, Ixora coccinea, Nyctanthes arbor-tristis, Polycythaemia rubra, Pongamia pinnata and Syzygium cumini, Carthamus tinctorius, Cucurbita maxima, Murraya koenigii, Leucas aspera, Plumbago indica and Psidium guajava.
Ethanolic extracts of most plants had phytochemicals, alkaloids,
glycosides, terpenoids, reducing sugars, saponins, tannins, flavonoids
and steroids.
Conclusions
These
plants could be used further for the isolation of pure compounds to be
used as complementary non-microbial antimicrobial medicines.
Keywords
- Ethnobotany;
- Antibacterial property;
- MDR bacteria;
- Phyto-extracts;
- Phytochemical analyses;
- Minimum bactericidal concentration
- Foundation Project: Supported by UGC, New Delhi (Grant No. 39-388/2010/SR).
- Peer reviewer
- Dr. Pawan K. Singh, FNAAS, FNA, Former VC at CSAU&T, Kanpur (UP), Present INSA Senior Scientist at Center of Advanced Studies, Department of Botany, Banaras Hindu University, Varanasi 2200, UP, India.
- Tel:
0542-2277705, 09415714584 - E-mail: pksalgae@yahoo.co.in
- Comments
- Methodology is universally accepted. Data were well presented. Since plant extracts have an array of compounds, the control of MDR bacteria was easy. Thus, plant extracts could be used as CAM. These 30 plants are used by Odishan ethnic tribes as traditional medicine.
- Details on Page 148
- Available online 21 Jul 2014
- Corresponding
author: Prof. Dr. Rabindra Nath Padhy, Central Research Laboratory, IMS
& Sum Hospital, Siksha ‘O’ Anusandhan University, Kalinga Nagar,
K-8, Bhubaneswar-751003, Odisha, India. Tel: +919437134982
Copyright © 2015 Asian Pacific Tropical Medicine Press. Published by Elsevier (Singapore) Pte Ltd. All rights reserved.
