Monday, 30 May 2016

Orange beverage ameliorates high-fat-diet-induced metabolic disorder in mice

Volume 24, June 2016, Pages 254–263


A low alcoholic orange beverage high in bioactive compounds was prepared.
Its effects were tested in high-fat-diet-induced metabolic disorder in mice.
Its long-term intake decreased plasma TAG, oxidized LDL and C-reactive protein levels.
Its consumption is beneficial for the metabolic consequences of obesity.


Metabolic syndrome (MetS) refers to a group of disorders that includes insulin resistance, central obesity, arterial hypertension and hyperlipidaemia. Regular consumption of bioactive compounds has consistently been associated with a reduced risk of these disorders. The aim of this study was to determine if an orange beverage with high concentrations of bioactive compounds (flavanones, carotenoids, melatonin, and ascorbic acid) and low alcohol content (<1%, v/v) improves metabolic parameters through modulation of oxidative stress, lipid profile and inflammatory response in a rodent model of high fat diet (HFD)-induced obesity. Mice with HFD-induced MetS were fed the orange beverage for 12 weeks (volume equivalent to 250 mL/day in human). Long-term intake of the orange beverage decreased plasma TAG, oxidized LDL and C-reactive protein levels. The present data provide evidence of a beneficial effect of orange beverage intake on some outcome parameters related to HFD-induced MetS.

Chemical compounds

  • Melatonin (PubChem CID: 896);
  • Ascorbic acid (PubChem CID: 54670067);
  • Ethanol (PubChem CID: 702);
  • Hesperidin (PubChem CID: 3594);
  • Naringenin-7-O-rutinoside (PubChem CID: 25244529);
  • Beta-cryptoxanthin (PubChem CID: 182237)


  • Fermented orange juice;
  • Bioactive compounds;
  • HFD;
  • Metabolic syndrome;
  • Antioxidant status;
  • Lipid profile


  • AI, atherogenic index;
  • AS, aqueous alcohol solution;
  • AUC, area under the curve;
  • CAT, catalase;
  • CTL, control;
  • CRP, C-reactive protein;
  • GPx, glutathione peroxidase;
  • GR, glutathione reductase;
  • GSH, reduced glutathione;
  • GSSG, oxidized glutathione;
  • HFD, high fat diet;
  • IPGTT, intraperitoneal glucose tolerance test;
  • MDA, malondialdehyde;
  • OB, orange beverage;
  • OJ, orange juice;
  • oxLDL, oxidized low-density lipoprotein;
  • PAC, plasma antioxidant capacity;
  • SOD, superoxide dismutase;
  • TC, total cholesterol
Corresponding author. Genoveva Berná Amorós, Departamento de Biología Molecular e Ingeniería Bioquímica, Universidad Pablo Olavide, Carretera de Utrera Km 1, 41013 Sevilla, Spain. Tel.: +34 954977943; fax: +34 954349813.