Volume 70, August 2015, Pages 272–279
Highlights
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- Antioxidant capacity of 11 species used in traditional medicine as anti-inflammatory.
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- Methods based on different mechanisms must be used to assess the antioxidant capacity.
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- Tibouchina kingii and Mimosa albida are potential sources of antioxidants.
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- Anti-inflammatory medicinal plants may serve as sources of natural antioxidants.
Abstract
Oxidative
stress is a major factor related to the development of inflammatory
diseases, which have traditionally been treated with medicinal plants.
Given this relationship, into the research of natural antioxidant
sources, the antioxidant capacity profiles of 11 species used in
traditional medicine as anti-inflammatories were determined. The
antioxidant capacity profile was investigated by using different
methodologies that consider electron transfer mechanisms (ET), such as
stabilization of the DPPH
radical, ferric reducing antioxidant power (FRAP), Trolox equivalent
antioxidant capacity (TEAC), and hydrogen atom transfer mechanisms (HAT)
such as oxygen radical absorption capacity (ORAC) and the inhibition of
lipid peroxidation of lipoproteins through the assessment of conjugated
dienes and thiobarbituric acid reactive substances (TBARS).
Additionally, the total polyphenol content by the Folin–Ciocalteu method
was determined, and the correlation between antioxidant capacity and
polyphenol content was estimated. The best amounts of total phenolics
and maximum values found in DPPH, FRAP, TEAC, and inhibition of lipid
oxidation tests were for extracts of Tibouchina kingii, Pseudelephantopus spiralis, and Mimosa albida. Correlation between polyphenol content with ET tests was observed (r2 > 0.85, p < 0.001),
explaining more than 72% of the antioxidant capacity of the extracts.
Additionally, a highly significant correlation was observed between the
ORAC and the TBARS tests, confirming that the antioxidant capacity is
due in part to transfer mechanisms involving hydrogen atoms. These
findings may confirm the interesting potential of these plants as a
valuable source of natural bioactive molecules.
radical, ferric reducing antioxidant power (FRAP), Trolox equivalent
antioxidant capacity (TEAC), and hydrogen atom transfer mechanisms (HAT)
such as oxygen radical absorption capacity (ORAC) and the inhibition of
lipid peroxidation of lipoproteins through the assessment of conjugated
dienes and thiobarbituric acid reactive substances (TBARS).
Additionally, the total polyphenol content by the Folin–Ciocalteu method
was determined, and the correlation between antioxidant capacity and
polyphenol content was estimated. The best amounts of total phenolics
and maximum values found in DPPH, FRAP, TEAC, and inhibition of lipid
oxidation tests were for extracts of Tibouchina kingii, Pseudelephantopus spiralis, and Mimosa albida. Correlation between polyphenol content with ET tests was observed (r2 > 0.85, p < 0.001),
explaining more than 72% of the antioxidant capacity of the extracts.
Additionally, a highly significant correlation was observed between the
ORAC and the TBARS tests, confirming that the antioxidant capacity is
due in part to transfer mechanisms involving hydrogen atoms. These
findings may confirm the interesting potential of these plants as a
valuable source of natural bioactive molecules.Keywords
- Anti-inflammatory medicinal plants;
- Antioxidant capacity;
- Electron transfer antioxidant tests;
- Hydrogen transfer antioxidant tests;
- Phenolic compounds
Copyright © 2015 Elsevier B.V. All rights reserved.
