Volume 170, 21 July 2015, Article number 9486, Pages 20-27
(Article)
a Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Cheong-ju, Chungbuk, South Korea
b College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, South Korea
c Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea
b College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, South Korea
c Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea
Abstract
Abstract Ethnopharmacological relevance Pseudolysimachion rotundum var. subintegrum (Speedwell, Plantaginaceae) is used as a traditional herbal medicine for treating bronchitis, cough and asthma in Korea, China,Russia, and Europe. Aim of the study In this study, we investigated the protective effects of the novel iridoid glycoside, piscroside C (compound 1) isolated from the methanolic extract of P. rotundum var. subintegrum against inflammatory responses using a cigarette smoke induced chronic obstructive pulmonary disease (COPD) and TNF-α-stimulated human airway epithelial NCI-H292 cells. Materials and methods The novel iridoid glycoside piscroside C was isolated from the methanolic extract of P. rotundum var. subintegrum. The chemical structure was established by NMR, HRESIMS, and optical rotation. In in vivo experiment, the mice received 1 h of cigarette smoke for 3 days. Piscroside C was administered to mice by oral gavage 1 h before cigarette smoke exposure for 3 days. In in vitro experiment, we evaluated the effect of piscroside C on proinflammatory mediators in H292 cells stimulated with TNF-α. Results Piscroside C significantly reduced the neutrophil influx, reactive oxygen species production, IL-6, TNF-α, and elastase activity in bronchoalveolar lavage fluid in COPD animals. In addition, piscroside C attenuated NF-κB and IκB phosphorylation, leading to reduced recruitment of inflammatory cells into the lung tissue. Consistent with the results of in vivo experiment, piscroside C significantly inhibited the expression of inflammatory cytokines (IL-6, IL-8 and IL-1β) by inhibiting NF-κB activation, as resulting decrease in the phosphorylation of IKKβ, IκBα and TAK1 in TNF-α-stimulated H292 cells. Conclusion These findings indicate that piscroside C effectively inhibits inflammatory responses, which is an important process in the development of COPD through suppression of IKK/NF-κB activation. Our study suggest that piscroside C might represent a useful therapeutic for the treatment of inflammatory airway disease. © 2015 Elsevier Ireland Ltd.
Author keywords
Cigarette smoke; Inflammation; NF-κB; Piscroside C; Pseudolysimachion rotundum var. subintegrum
Indexed keywords
EMTREE drug terms: antiinflammatory agent; cigarette smoke; elastase; I kappa B; I kappa B alpha; I kappa B kinase beta; immunoglobulin enhancer binding protein; interleukin 1beta; interleukin 6; interleukin 8; iridoid; piscroside C; plant extract; Pseudolysimachion rotundum var. subinegrum extract; reactive oxygen metabolite; transforming growth factor beta activated kinase 1; tumor necrosis factor alpha; unclassified drug
EMTREE medical terms: animal experiment; animal model; antiinflammatory activity; Article; chronic obstructive lung disease; controlled study; electrospray mass spectrometry; enzyme activation; enzyme activity; human; human cell; in vitro study; in vivo study; lung lavage; lung parenchyma; medicinal plant; neutrophil; nonhuman; nuclear magnetic resonance spectroscopy; optical rotation; plant leaf; Plantaginaceae; protein phosphorylation; Pseudolysimachion rotundum var. subinegrum; respiratory epithelium; respiratory tract inflammation
Species Index: Animalia; Mus; Plantaginaceae; Veronica
