Volume 67, May 2015, Pages 249–256
Solvent effect on total phenolic contents, antioxidant, and antibacterial activities of Matricaria pubescens
Highlights
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- The extraction of polyphenol compounds by using various solvents.
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- The determination of polyphenols, flavonoids, and condensed tannins.
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- The determination of the antioxidant activity using different methods.
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- The determination of the antibacterial activity.
Abstract
Matricaria Pubescens
(Asteraceae) is a well known medicinal plant used in the south west of
Algeria against several diseases. This study was devoted to the
determination of polyphenols, flavonoids, and condensed tannins contents
of M. pubescens after their extraction using various
solvents with different polarities (distilled water, aqueous acetone
(50%), aqueous methanol (50%), aqueous ethanol (50%), acetone (100%),
methanol (100%), and ethanol (100%)), as well as the determination of
the antioxidant and the antibacterial activities of all obtained
extracts. The results obtained indicate that the aqueous methanol (50%)
and the aqueous ethanol (50%) extracts presented the highest contents of
polyphenols. The antioxidant activity was evaluated using ferric
reducing power, DPPH radical scavenging activity, ABTS radical
scavenging activity, oxygen radical absorbance capacity (ORAC) and
hydroxyl radical scavenging capacity (HOSC). The results showed that all
extracts of M. pubescens, prepared using different
solvent, have diverse antioxidant capacities, however aqueous methanol
(50%), aqueous ethanol (50%) and aqueous acetone (50%) exhibited the
highest antioxidant capacity. Extracts of M. pubescens exhibited an inhibiting capacity against the tested strains except: Escherichia coli ATCC 25922 and Acinetobacter baumannii
897. The aqueous methanol (50%) and the aqueous ethanol (50%) extracts
were most effective; the greatest zone of inhibition was obtained with
the aqueous ethanol (50%) extract against Methicillin-resistant Staphylococcus aureuscStaphylococcus aureusc. The minimum inhibitory concentration (MIC) with the value of 0.78 mg/mL obtained against A. baumannii
610 using the aqueous methanol (50%) extract indicate that this plant
is a potential natural antimicrobial agent. These biological activities
could support the traditional use of M. pubescens.
Keywords
- Matricaria pubescens;
- Polyphenols;
- Flavonoids;
- Condensed tannins;
- Antioxidant Activity;
- Antibacterial activity
Copyright © 2015 Elsevier B.V. All rights reserved.
