Pharm Biol. 2016 Jan 5:1-5. [Epub ahead of print]
Abstract
Context
The increasing problem of drug-resistant strains has led to the failure
of current treatment regimens of Helicobacter pylori (HP) infection.
Recently, a new treatment strategy has been developed to overcome the
problem by using natural products in combination with antibiotics to
enhance the treatment efficacy. Objective The antimicrobial combinatory
effect of the aqueous extract of Hibiscus
sabdariffa L. (Malvaceae) (AEHS) with antibiotics (clarithromycin, CLA;
amoxicillin, AMX; metronidazole, MTZ) has been evaluated in vitro
against HP strains. Materials and methods Hibiscus
calyces (35 g) were brewed in 250 mL of boiled water for 30 min, and
minimum inhibitory concentrations (MICs) were determined by agar
dilution method. The checkerboard assay was used to evaluate the
antimicrobial combinatory effect according to the sum of fractional
inhibitory concentration (∑FIC) indices. Results In this study, AEHS
exerted remarkable bacteriostatic effect against all HP strains tested
with MICs values ranging from 9.18 to 16.68 μg/mL. Synergy effect of
AEHS with CLA or MTZ was obtained against four of seven HP strains
tested with ∑FIC ranging from 0.21 to 0.39. The additive effect of AEHS
with AMX was obtained against five of seven HP strains tested with ∑FIC
ranging from 0.61 to 0.91. Conclusion This study presents AEHS as a
potent therapeutic candidate alone, or in combination with antibiotics
for the treatment of HP infection.
