World Applied Sciences Journal 30 (11): 1599-1604, 2014
ISSN 1818-4952
© IDOSI Publications, 2014
DOI: 10.5829/idosi.wasj.2014.30.11.14220
Corresponding Author: Nina Georgievna Machavariani, Gause Institute of New Antibiotics, Russian Academy of
Medical Sciences, Bolshaya Pirogovskaya Street, 11, 119021, Moscow, Russian Federation
1599
Nina Georgievna Machavariani, Tatyana Dmitrievna Ivankova,
Olga Nikolaevna Sineva and Larissa Petrovna Terekhova
Gause Institute of New Antibiotics, Russian Academy of Medical Sciences,
Bolshaya Pirogovskaya Street, 11, 119021, Moscow, Russian Federation
Abstract: Endophytic bacteria are microorganisms that colonize the internal tissues of the plant without causing
any external sign of infection or negative effect on their host. In the present study, endophytic actinomycetes
were isolated from leaves of medicinal plants collected in Moscow and Moscow region. To isolate
actinomycetes we used a method developed by us on the basis of known techniques. The main modification
consisted in the pretreatment of leaves with solutions of biologically active substances of plant origin -
heteroauxin and zircon. Using the modification method of this study helps to increase numbers of rare
actinomycetes isolated from the medicinal plants, which are potentially unique source of novel bioactive
metabolites for medicinal and biotechnological practice.
Key words: Endophytic actinomycetes Actinobacteria Zircon Heteroauxin Medicinal plants
