Determination of marker compounds in combined herbal extract-based anti-prostatitis herbal product

Research Journal of Pharmaceutical, Biological and Chemical Sciences

Volume 7, Issue 2, 2016, Pages 423-429

Beloborodov, V.^b, 

Voskoboynikova, I.^a, 

Kolkhir, V.^a, 

Savvateev, A.^b, 

Zakharova, N.^a

 

^a  Department of Experimental and Clinical Pharmacology, National Institute of Therapeutic and Aromatic Plants (VILAR), 7 Grina Str., Moscow, Russian Federation
^b  Department of Organic Chemistry, I.M. Sechenov First Moscow State Medical University, Bldg.2, 8 Trubetskaya Str., Moscow, Russian Federation 

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Abstract

Herbal product (Prostanorm) is a multi-component herbal drug containing extract of a mixture of St. John's wort (Hypericum perforatum L.), golden rod (Solidago Canadensis L.), licorice root (Glycyrrhiza glabra L.), Echinacea purpurea (L.) Moench rhizome and roots in equal quantitites. The drug is approved in Russia for treatment of chronic prostatitis in men. Reliable standardization of the product requires quantitative analysis of the main components of the herbal extract. We have previously developed a method of solid phase extraction combined with HPLC for identification and quantitative analysis of marker substances of each herbal component of product, and showed a possibility of their use as markers for control of components' content in the product. Here we describe the applicability of this approach for analysis of the total Prostanorm product. The method includes solid phase extraction on Discovery DSC-18 LT SPE cartridge and subsequent reverse phase HPLC. Drug aliquots were applied onto SPE cartridge, and highlyionic substances (caftaric acid, chlorogenic acid, caffeic acid, cichoric acid, 2-hydroxy-cinnamic acid, rutin, hyperoside) were eluted by 0.2% orthophosphoric acid (fraction 1), while less ionic substances (quercetin, Kempferol and glycyrrhizinic acid) were subsequently eluted by ethanol (fraction 2). Both fractions were subjected to two-stage gradient reverse phase HPLC. Optimal chromatographic conditions for separation of marker compounds were established. Method specificity, reproducibility and repeatability were confirmed. The following levels of marker substances (in mg/ml) were found in Prostanorm using the developed assay method: Caffeic acid0.21, caftaric acid 0.83, chlorogenic acid 1.34, chicoric acid 3.25, glycyrrhizinic acid 14.98, 2-hydroxy-cinnamic acid 0.47, hyperoside 1.94, kempferol 0.08, quercetin 1.18 and rutin 2.47. SPE/HPLC method was shown to be appropriate for the quality control of the Prostanorm liquid extract.

Author keywords

Herbal product (Prostanorm); HPLC; Marker compounds; Quantitative analysis; Solid phase extraction

ISSN: 09758585Source Type: Journal Original language: English

Document Type: Article

Publisher: Research Journal of Pharmaceutical, Biological and Chemical Sciences

References (21)

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  Kolkhir, V.; Department of Experimental and Clinical Pharmacology, National Institute of Therapeutic and Aromatic Plants (VILAR), 7 Grina Str., Russian Federation
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