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Tuesday, 3 May 2016

Molecular surveillance of Theileria equi and Anaplasma phagocytophilum infections in horses from Ukraine, Poland and Slovakia

Veterinary Parasitology
Volume 215, 15 January 2016, Pages 35–37
Short communication



doi:10.1016/j.vetpar.2015.10.025
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Highlights

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A survey was undertaken to assess the prevalence of T. equi and A. phagocytophilum in Ukraine, Poland and Slovakia.
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The prevalence of T. equi and A. phagocytophilum among 215 horses was 13.95% and 1.4%, respectively.
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Horses from Poland and Ukraine tested positive for T. equi.
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Horses from Poland and Slovakia tested positive for A. phagocytophilum.
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No dual infections with protozoan and bacterial species were found.

Abstract

A survey was undertaken to assess the prevalence of Theileria equi and Anaplasma phagocytophilum in some regions of Ukraine, Poland and Slovakia. Using a specific PCR assays, blood samples from 215 horses were tested. The prevalence of T. equi and A. phagocytophilum infection was 13.95% and 1.4%, respectively. BLAST analysis showed the isolates closest to the T. equi 18S rRNA and A. phagocytophilum msp4 gene sequences in GenBank with a similarity of ≥99%. No significant association was found between the T. equi PCR positivity and the age or sex of the horses. There was a significant association between the origin of horses and T. equi—PCR positivity. No significant association was found between the A. phagocytophilum—PCR positivity and the age, sex or origin.

Keywords

  • Anaplasma phagocytophilum;
  • Theileria equi;
  • Horse;
  • Poland;
  • Slovakia;
  • Ukraine
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Copyright © 2015 Elsevier B.V. All rights reserved.
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