Original Research Article
- a Faculty of Natural Sciences, Vytautas Magnus University, Kaunas, Lithuania
- b Kaunas Botanical Garden of Vytautas Magnus University, Sector of Medicinal Plants, Kaunas, Lithuania
- c Faculty of Pharmacy, Lithuanian University of Health Sciences, Kaunas, Lithuania
- Received 15 October 2013, Accepted 31 October 2013, Available online 21 March 2014
Abstract
Purpose
Willow
herb has been traditionally used in folk medicine and currently it is a
potential raw material for production of phytopharmaceuticals. The aim
of this work was to determine the highest amount of flavonoids and the
highest radical scavenging activity of willow herb, which was collected
in different vegetation phases (intensive growing, bud, massive
blooming, ripening of fruits (seeds) and the end of vegetation) and in
different parts of the plant (blooms, leaves and stems).
Material/methods
Raw
material was collected at Kaunas Botanical garden of Vytautas Magnus
University. Willow herb was extracted using methanol/water mixture
(75/25 v/v, %). Methanolic extracts were purified using solid-phase
extraction. For determination of the radical scavenging activity of
compounds the HPLC system with the on-line post-column DPPH
(1,1-diphenyl-2-picrylhydrazyl) radical reaction detection was used.
Results
Five
flavonoids were identified and their quantitative distribution and
radical scavenging activity were evaluated. The highest total amount of
flavonoids and radical scavenging activity were determined in willow
herb collected during the massive blooming phase (11.12 ± 0.34 mg/g and
8.71 ± 0.29 mg/g, respectively).
Conclusions
The
highest amount of flavonoids and radical scavenging activity was
determined for raw material collected during the massive blooming phase.
Evaluation of different parts of the plant during the massive blooming
phase revealed that the highest amount of flavonoids and radical
scavenging activity are characteristic for blooms of the plant.
Keywords
- Chamerion angustifolium (L.);
- Flavonoids;
- DPPH;
- Vegetation phases;
- Radical scavenging activity
Copyright © 2014 Medical University of Bialystok. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.
