Volume 106, October 2015, Pages 7–11
Abstract
The
isolation and structural elucidation of a macrocyclic alkaloid,
characterized by the presence of a 13-membered macrolactam ring
containing a spermidine unit N-linked to a benzoyl group is hereby reported. The structure of this previously unknown spermidine alkaloid isolated from Gymnosporia arenicola (Celastraceae) leaves has been elucidated by 1H and 13C
NMR spectroscopy (including bidimensional analysis) and further
characterized by high-resolution mass spectrometry and polarimetry. A
route for the biosynthesis of this new bioactive macrocycle is proposed
and the cytotoxicity of the compound was evaluated against two ATCC cell
lines – one normal-derived (MCF10A) and one cancer-derived cell line
(MCF7) – using the MTT assay. The alkaloid revealed to be non-cytotoxic
against both cell lines. The IC50 values from the cells were also determined.
Keywords
- Gymnosporia arenicola;
- Celastraceae;
- Macrolactam ring;
- Spermidine alkaloid;
- Structural characterization
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