Volume 10, Issue 8, 11 August 2015, Article number e0135721
a
Natural Health Products and Metabolic Disease Laboratory and
Montreal Diabetes Research Center, Department of Pharmacology,
Université de Montréal, Montreal, QC, Canada
b Canadian Institutes of Health Research Team, Aboriginal Antidiabetic Medicines, Montreal, QC, Canada
c Institute of Nutraceuticals and Functional Foods, Université Laval, Quebec, QC, Canada
b Canadian Institutes of Health Research Team, Aboriginal Antidiabetic Medicines, Montreal, QC, Canada
c Institute of Nutraceuticals and Functional Foods, Université Laval, Quebec, QC, Canada
Abstract
We evaluated and
compared the antidiabetic potential and molecular mechanisms of 17 Cree
plants' ethanol extracts (EE) and hot water extracts (HWE) on glucose
homeostasis in vitro and used metabolomics to seek links with the
content of specific phytochemicals. Several EE of medical plants
stimulated muscle glucose uptake and inhibited hepatic G6Pase activity.
Some HWE partially or completely lost these antidiabetic activities in
comparison to EE. Only R. groenlandicum retained similar potential
between EE and HWE in both assays. In C2C12 muscle cells, EE of R.
groenlandicum, A. incana and S. purpurea stimulated glucose uptake by
activating AMP-activated protein kinase (AMPK) pathway and increasing
glucose transporter type 4 (GLUT4) expression. In comparison to EE, HWE
of R. groenlandicum exhibited similar activities; HWE of A. incana
completely lost its effect on all parameters; interestingly, HWE of S.
purpurea activated insulin pathway instead of AMPK pathway to increase
glucose uptake. In the liver, for a subset of 5 plants, HWE and EE
activated AMPK pathway whereas the EE and HWE of S. purpurea and K.
angustifolia also activated insulin pathways. Quercetin-3-O-galactoside
and quercetin 3-O-α-L-arabinopyranoside, were successfully identified by
discriminant analysis as biomarkers of HWE plant extracts that
stimulate glucose uptake in vitro. More importantly, the latter compound
was not identified by previous bioassay-guided fractionation.
Copyright: © 2015 Shang et al.
Indexed keywords
EMTREE drug terms: Abies balsamea extract; alcohol;
Alnus incana extract; flavone derivative; Gaultheria hispidula extract;
glucose; glucose 6 phosphatase; glucose transporter 4; hot water;
hydroxymethylglutaryl coenzyme A reductase kinase; hyperin; insulin; Juniperus communis
extract; Kalmia angustifolia extract; Larix laricina extract;
Lycopodium clavatum extract; Picea glauca extract; Picea mariana
extract; Pinus banksiana extract; plant extract; Populus balsamifera
extract; quercetin 3 o alpha arabinopyranoside; Rhododendron
groenlandicum extract; Rhododendron tomentosum extract; Salix planifolia
extract; Sarracenia purpurea extract; Sorbus decora extract;
unclassified drug; Vaccinium vitis idaea extract
EMTREE medical terms: Abies balsamea; alder; Alnus
incana; animal cell; antidiabetic activity; Article; bioassay; black
spruce; C2C12 cell line; Canada; controlled study; drug determination;
drug mechanism; enzyme activation; enzyme activity; ethnobotany;
fractionation; Gaultheria; Gaultheria hispidula; glucose homeostasis;
glucose transport; hepatoma cell; in vitro study; insulin metabolism; Juniperus;
Kalmia angustifolia; Larix; Larix laricina; lingonberry; Lycopodium;
medicinal plant; metabolomics; muscle cell; nonhuman; phytochemistry;
pine; Pinus banksiana; Populus balsamifera; protein analysis; protein
expression; rat; Rhododendron; Rhododendron groenlandicum; Rhododendron
tomentosum; Salix planifolia; Sarracenia purpurea; signal transduction;
Sorbus; Sorbus decora; white spruce; willow
Chemicals and CAS Registry Numbers: alcohol, 64-17-5;
glucose, 50-99-7, 84778-64-3; glucose 6 phosphatase, 9001-39-2; glucose
transporter 4, 188071-24-1; hydroxymethylglutaryl coenzyme A reductase
kinase, 172522-01-9, 72060-32-3; hyperin, 482-36-0; insulin, 9004-10-8
ISSN: 19326203
CODEN: POLNCSource Type: Journal
Original language: English
DOI: 10.1371/journal.pone.0135721Document Type: Article
Publisher: Public Library of Science
© Copyright 2015 Elsevier B.V., All rights reserved.