Endophytic fungi from tropical ethnoveterinary plants and their antibacterial efficacy against Pasteurella multocida capsular type a strain

Revista de Biologia Tropical

Volume 64, Issue 2, June 2016, Pages 733-745

Open Access

[Hongos endófitos de plantas tropicales etnoveterinarias y su eficacia antibacteriana contra cepas capsulares de Pasteurella multocida tipo a]

Nath, A. , 

Joshi, S.R. 

 

Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, India

View references (42)

Abstract

Pasteurella multocida is an important veterinary pathogen causing infections in animals and birds. Nowadays, different reports have described the severity of infections, increasing resistance of micro-organisms to antibiotics, and the contribution of ethnoveterinary practices towards the treatment of various ailments of animals. The aim of the present study was to investigate the antibacterial efficacy of the ethanolic extracts of endophytic fungi against P. multocida Capsular Type A strains. A total of six endophytic fungi were isolated from two tropical ethnoveterinary plants: Garcinia xanthochymus H. and Polygonum chinense L. The ethanolic extracts of the endophytic fungi were subjected to in vitro antimicrobial activity by the well diffusion method. Besides, we evaluated the treatment of mice with the potent fungal extract and observed the effects in different organs under electron microscopy. Our results showed that four fungi had antimicrobial activity against the selected pathogen. The best antibacterial activity was showed by the extract of the endophytic fungi, Glomerella magna isolated from G. xanthochymus, with a minimum inhibitory concentration of 46.9 µg/mL and minimum bactericidal concentration of 750 µg/mL. Treatment of mice with the potent fungal extract caused a considerable inhibitory effect on the pathogen growth in vital organs, results that was also confirmed by histopathological studies made by scanning electron microscopy. The present findings indicated that the endophytic fungi G. magna has the potential to provide an effective treatment against infections caused by Pasteurella multocida. However, the isolation of bioactive components needs further investigation. © 2016, Universidad de Costa Rica. All rights reserved.

Author keywords

Antibacterial; Endophytic fungi; Ethnoveterinary plants; Pasteurella multocida; Scanning electron microscopy

ISSN: 00347744Source Type: Journal Original language: English

DOI: 10.15517/rbt.v64i2.18584Document Type: Article

Publisher: Universidad de Costa Rica

References (42)

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  Joshi, S.R.; Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, India; email:srjoshi2006@yahoo.co.in
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