industrial Crops and Products
Volume 47, May 2013, Pages 126-131
a
Laboratório Nacional de Energia e Geologia, I.P., Estrada do Paço do Lumiar, 22, 1649-038 Lisboa, Portugal
b Sociedade Portuguesa de Fitoquímica e Fitoterapia, Rua da Sociedade Farmacêutica, 18, 1169-075 Lisboa, Portugal
c Laboratorio de Inmunoquímica II, Departamento de Inmunología, Escuela Nacional de Ciências Biológicas, Casco de S.Tomás 11340, Mexico
b Sociedade Portuguesa de Fitoquímica e Fitoterapia, Rua da Sociedade Farmacêutica, 18, 1169-075 Lisboa, Portugal
c Laboratorio de Inmunoquímica II, Departamento de Inmunología, Escuela Nacional de Ciências Biológicas, Casco de S.Tomás 11340, Mexico
Abstract
The aim of the present study was the search for the industrial exploitation potential of 15 Hypericum species crops from Portugal. Although Hypericum perforatum is well known worldwide, scarce studies have been published of Hypericum spp. identified in Portugal.Extracts
from 15 Hypericum species were screened for its antimicrobial
activities against 2 Gram- and 2 Gram+ bacteria, 4 non-tuberculous
Mycobacterium species, a reference strain H37Rv and 4
drug-resistant strains of Mycobacterium tuberculosis, as well as 4
drug-resistant clinical isolates.In terms of Gram - standards, H.
humifusum and H. elodes were the most active against Agrobacterium
tumefasciens, with MIC of 2.5 μg/mL. H. elodes and H. hircinum subsp.
majus extracts were the most active against MDR-TB strains and isolates,
with MIC of 25-100 μg/mL and both exhibited significant effect against
MDR-TB clinical isolates. With the exception of H. androsaemum and H.
linarifolium all Hypericum species were active against Staphylococcus
aureus, the H. perfoliatum and H. elodes at the level of 6 and 12 μg/mL,
respectively, and none showed activity on E. coli.Reference compounds
isolated from H. perforatum were also tested and might contribute to the
activities observed. The profile of the 15 Hypericum spp. as effective
antimicrobial therapy against multidrug resistant pathogens is now
available, providing scientific validation on a few available
ethnopharmacological data resources.This study may be a starting point
for the research on the role of various Hypericum species in integrative
medicine for infection control of S. aureus and MDR-MTB. Hypericum
species may also constitute a source of new leads towards the discovery
of either new candidates and biologically active compounds for
pharmaceutical interest, for the treatment of multidrug resistant
diseases. © 2013 Elsevier B.V.
Author keywords
Agrobacterium tumefasciens; Antibacterial activity;
Antimycobacterial activity; Hyperforin; Hypericaceae; Hypericin;
Hypericum species; Multidrug-resistant Mycobacterium tuberculosis;
Mycobacterium species; Pseudohypericin; Staphylococcus aureus
Indexed keywords
Agrobacterium; Anti-bacterial activity;
Antimycobacterial activity; Hyperforins; Hypericaceae; Hypericins;
Hypericum species; Mycobacterium species; Mycobacterium tuberculosis;
Pseudohypericin; Staphylococcus aureus
Engineering controlled terms: Bacteria; Bioactivity; Drug therapy; Strain; Tubes (components)
Engineering main heading: Essential oils
GEOBASE Subject Index: antibiotic resistance; antimicrobial activity; bacterium; biological control; ethnobotany; medicine; plant extract
Regional Index: Portugal
Species Index: Agrobacterium; atypical mycobacterium;
Bacteria (microorganisms); Clusiaceae; Hypericum; Hypericum androsaemum;
Hypericum elodes; Hypericum hircinum; Hypericum humifusum; Hypericum
perfoliatum; Hypericum perforatum; Mycobacterium; Mycobacterium
tuberculosis; Rhizobium; Staphylococcus aureus
