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Friday, 13 May 2016

The isolation and characterization of actinobacteria from dominant benthic macroinvertebrates endemic to Lake Baikal

Volume 61, Issue 2, 1 March 2016, Pages 159-168


a  Institute of Biology at Irkutsk State University, Irkutsk, Russian Federation
b  Helmholtz Institute for Pharmaceutical Research Saarland, Saarbrucken, Germany 

Abstract

The high demand for new antibacterials fosters the isolation of new biologically active compounds producing actinobacteria. Here, we report the isolation and initial characterization of cultured actinobacteria from dominant benthic organisms’ communities of Lake Baikal. Twenty-five distinct strains were obtained from 5 species of Baikal endemic macroinvertebrates of amphipods, freshwater sponges, turbellaria worms, and insects (caddisfly larvae). The 16S ribosomal RNA (rRNA)-based phylogenic analysis of obtained strains showed their affiliation to Streptomyces, Nocardia, Pseudonocardia, Micromonospora, Aeromicrobium, and Agromyces genera, revealing the diversity of actinobacteria associated with the benthic organisms of Lake Baikal. The biological activity assays showed that 24 out of 25 strains are producing compounds active against at least one of the test cultures used, including Gram-negative bacteria and Candida albicans. Complete dereplication of secondary metabolite profiles of two isolated strains led to identification of only few known compounds, while the majority of detected metabolites are not listed in existing antibiotic databases. © 2015, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i.
ISSN: 00155632 CODEN: FOMIASource Type: Journal Original language: English
DOI: 10.1007/s12223-015-0421-zDocument Type: Article
Publisher: Springer Netherlands
Funding Details
Sponsor: Deutscher Akademischer Austauschdienst
Acronym; Sponsor: DAAD; Deutscher Akademischer Austauschdienst
Number; Acronym; Sponsor: 15-04-06685; RFBR; Deutscher Akademischer Austauschdienst
Number; Acronym; Sponsor: N 14-04-00501; RFBR; Deutscher Akademischer Austauschdienst
Number; Acronym; Sponsor: N 14-14-00400; RSF; Deutscher Akademischer Austauschdienst

  Axenov-Gribanov, D.; Institute of Biology at Irkutsk State University, Russian Federation; email:denis.axengri@gmail.com
© Copyright 2016 Elsevier B.V., All rights reserved.